Should The Plasma Membrane Study: Hcl, Naoh, Sds
Biological Membrane Study: HCl, NaOH, SDS
Objective and Hypothesis: The objective of this lab is to determine the effects of SDS, HCl, and NaOH on red cabbage cell membrane. Prior knowledge can tell us that the red cabbage is a red-purple color due to a pigment called anthocyanin. This is what we will be testing in the experiment. We also know that HCl is a strong acid (very low pH) and that NaOH is a strong base (very high pH). From information learned in this course (lecture/lab) we can assume that both a very high and low pH will have adverse effect on the plasma membrane. SDS (Sodium dodecyl sulfate) is a detergent. From our back-round information we can learn that soaps and detergents tend to solubilize the lipids of the cell membrane causing cells to lyse. So overall we can assume that all three are going to cause the plasma cell membrane of the red cabbage to break down. Which one, HCl/NaOH/SDS, will have the most adverse effect on the plasma membrane? I believe that the acid will have the most effect, then the base, then the …show more content…
The first step of the experiment was to determine the amount of each (acid, base, soap) we would need to use to obtain the desired concentrations. We used the formula P x C = P x C.
2. Next we prepare the red cabbage leaf discs buy using a hole puncher to punch out holes in the cabbage. We need 4 leaf discs per test tube, therefore we needed a total of 52 discs. Once the discs were made they were allowed to stay in a beaker of distilled water for about 10 minutes.
3. Next we tested the plant cell membrane integrity. We prepare 4mL test tubes containing: 4 discs, dH20, and a calculate amount of either HCl, NaOH, or SDS (refer to step 1-will also be in results). The discs then sat for 30-35 minutes.
4. Finally, after the waiting period of 30-35 minutes we removed 3mL of solution to a clean test tube. We then used these test tubes to determine a range of wavelengths that we used to calculate lambda max for each
Objective and Hypothesis: The objective of this lab is to determine the effects of SDS, HCl, and NaOH on red cabbage cell membrane. Prior knowledge can tell us that the red cabbage is a red-purple color due to a pigment called anthocyanin. This is what we will be testing in the experiment. We also know that HCl is a strong acid (very low pH) and that NaOH is a strong base (very high pH). From information learned in this course (lecture/lab) we can assume that both a very high and low pH will have adverse effect on the plasma membrane. SDS (Sodium dodecyl sulfate) is a detergent. From our back-round information we can learn that soaps and detergents tend to solubilize the lipids of the cell membrane causing cells to lyse. So overall we can assume that all three are going to cause the plasma cell membrane of the red cabbage to break down. Which one, HCl/NaOH/SDS, will have the most adverse effect on the plasma membrane? I believe that the acid will have the most effect, then the base, then the …show more content…
The first step of the experiment was to determine the amount of each (acid, base, soap) we would need to use to obtain the desired concentrations. We used the formula P x C = P x C.
2. Next we prepare the red cabbage leaf discs buy using a hole puncher to punch out holes in the cabbage. We need 4 leaf discs per test tube, therefore we needed a total of 52 discs. Once the discs were made they were allowed to stay in a beaker of distilled water for about 10 minutes.
3. Next we tested the plant cell membrane integrity. We prepare 4mL test tubes containing: 4 discs, dH20, and a calculate amount of either HCl, NaOH, or SDS (refer to step 1-will also be in results). The discs then sat for 30-35 minutes.
4. Finally, after the waiting period of 30-35 minutes we removed 3mL of solution to a clean test tube. We then used these test tubes to determine a range of wavelengths that we used to calculate lambda max for each