Biol 1107.103 (Tuesday 4-7)
The purpose of this lab is to be able to trace the flow of blood through both the mammalian and the shark heart. Learn all the major structures of the body systems of the shark and functions of certain structures as well as for the rat. Understand the terms of retroperitoneal, ovoviviparous, oviparous, and viviparous. Know which vessels and heart parts contain what “kind” of blood (oxygenated or deoxygenated), and perform a good dissection.
II. Materials and Methods
A Photographic Atlas
Ashoka (For assistance)
Methods and Procedures
1. One person of each pair gets a shark from the box assigned to your lab. 2. Take the bagged shark into the prep room or sink at the end of your table, Open the bag over the sink and pour the preservative as you run water from the tap. 3. Run tap water over the shark and rinse out the bag in which the shark will be stored. 4. Put the shark in a specimen tray and bring it back out to begin your study. 5. Learn about the external anatomy of the shark. 6. Begin your dissection and study of the shark’s internal anatomy. 7. Follow your lab assistant’s instructions as to the cuts you should make. 8. As you work your way through each of the body systems, identify the following structures and function. 9. Digestive System: peritoneum, stomach, small intestine, colon and rectum, rectal gland, cloaca, liver, pancreas, spleen, and gall bladder. 10. Urogenital System: kidneys, male archinephric ducts, testes, ovaries, uterus. 11. Circulatory System: sinus venosus, 1 atrium, 1 ventricle, conus arteriosus, ventral aorta, afferent branchial arteries, efferent branchial arteries, dorsal aorta,
Cited: Ratcliff, Rachel. 2010. General Biology II Laboratory Manual. Organ Systems of Squalus acanthias (shark) and Rattus norvegicus (rat). Dissection Experiments. 89-100.