SCI250 Week 1 Chapter 3 Quiz
SCI250 Week 1 Chapter 3 Staining Lab Quiz
Section: Multiple Choice
1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids?
A. Gram stain
B. Schaeffer-Fulton stain
C. Acid-fast stain
D. Lipidialar stain
E. Spore Stain
2. Which of the following stains is used to classify microorganisms based on their cell wall content?
A. Capsular stain
B. Gram stain
C. Spore stain
D. Negative stain
E. Methylene blue
3. In the Gram stain, Gram-positive bacteria stain with a _________ color due to the incorporation of the _________ dye into their cell wall.
A. purple, safranin
B. purple, crystal violet
C. red, safranin
D. red, crystal violet
4. What is the function of the mordant iodine in a Gram staining procedure?
A. A mordant causes the crystal violet to run.
B. A mordant fixes the bacteria to the slide.
C. A mordant keeps dye attached to an object.
D. All of the above
5. What is the order of reagents used in the Gram stain?
A. crystal violet, iodine, safranin, alcohol
B. alcohol, crystal violet, iodine, safranin
C. iodine, crystal violet, safranin, alcohol
D. crystal violet, iodine, alcohol, safranin
E. crystal violet, safranin, alcohol, iodine
6. Following a Gram stain you observe clear, glassy areas inside the bacterial cells. What does this suggest? Which stain would you use to confirm this?
A. A simple stain
B. A flagellar stain
C. Another Gram stain
D. An endospore stain
E. An acid-fast stain
7. The presence of a capsule around bacterial cells usually indicates their increased disease-causing potential and resistance to disinfection. Capsules are generally viewed by ________
A. spore staining.
B. scanning electron microscopy.
C. Gram staining.
D. Ziehl-Neelsen staining.
E. negative staining.
Section: True or False
8. Fluorescent microscopes use an ultraviolet (UV) light source and fluorochromes as stains.
True
False
9. Older, pure cultures of Gram-positive organisms can
Section: Multiple Choice
1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids?
A. Gram stain
B. Schaeffer-Fulton stain
C. Acid-fast stain
D. Lipidialar stain
E. Spore Stain
2. Which of the following stains is used to classify microorganisms based on their cell wall content?
A. Capsular stain
B. Gram stain
C. Spore stain
D. Negative stain
E. Methylene blue
3. In the Gram stain, Gram-positive bacteria stain with a _________ color due to the incorporation of the _________ dye into their cell wall.
A. purple, safranin
B. purple, crystal violet
C. red, safranin
D. red, crystal violet
4. What is the function of the mordant iodine in a Gram staining procedure?
A. A mordant causes the crystal violet to run.
B. A mordant fixes the bacteria to the slide.
C. A mordant keeps dye attached to an object.
D. All of the above
5. What is the order of reagents used in the Gram stain?
A. crystal violet, iodine, safranin, alcohol
B. alcohol, crystal violet, iodine, safranin
C. iodine, crystal violet, safranin, alcohol
D. crystal violet, iodine, alcohol, safranin
E. crystal violet, safranin, alcohol, iodine
6. Following a Gram stain you observe clear, glassy areas inside the bacterial cells. What does this suggest? Which stain would you use to confirm this?
A. A simple stain
B. A flagellar stain
C. Another Gram stain
D. An endospore stain
E. An acid-fast stain
7. The presence of a capsule around bacterial cells usually indicates their increased disease-causing potential and resistance to disinfection. Capsules are generally viewed by ________
A. spore staining.
B. scanning electron microscopy.
C. Gram staining.
D. Ziehl-Neelsen staining.
E. negative staining.
Section: True or False
8. Fluorescent microscopes use an ultraviolet (UV) light source and fluorochromes as stains.
True
False
9. Older, pure cultures of Gram-positive organisms can