11. Amylase catabolizes starch polymers into smaller subunits. Most organisms use these saccharides as a food source and to store energy. Amylase can be found in the saliva of humans and other mammals. (starch > sugar)…
When carbohydrates are consumed during a meal, catabolism originates in the mouth. The salivary enzyme α-amylase breaks down the carbohydrates through the hydrolysis of the α1->4 glycosidic bonds. This is followed by the further breakdown of the complex polysaccharides in the small intestine down to monosaccharides units in order for the glucose to be absorbed directly into the bloodstream.…
By having a control in this experiment, we can see the changes with the presence of amylase. Lugol’s regent (I2Kl) changes color in the presence of starch, which is the control group that we have setup that demonstrates what would happen naturally, with starch and Lugol’s regent (I2Kl). The control group will have a distinct comparison available for us. As amylase, an enzyme that catalyzes the breakdown of the starch into glucose, which will have no reaction with Lugol’s regent (I2Kl) as well as no color change.…
Amylase- an enzyme, found chiefly in saliva and pancreatic fluid, that converts starch and glycogen into simple sugars.…
Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature, pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions, which would otherwise progress more slowly.(Background Information; pg. 1) pH is a measurement of the acidity or alkalinity (base) of a solution. When the liver got mixed with H2O2 , the first time the chemical reaction was fast, the second time the reaction was slow and the last try was very fast. Temperature is the degree or intensity of heat present in a substance or object. When the temperature of the liver changed from freezing to very hot to room…
1) The purpose of this lab was to determine the rate of enzyme activity under variety of different conditions, such as, different amount of drops of enzymes and different temperature of water. The class measured the pressure in the test tube during the reaction of the substance with, 1.5 ml of H2O2, 1.5ml of H2O and different amounts of enzyme drops, to determine how much oxygen gas is produced during the reaction since the pressure of the test tube will get higher as more oxygen gas is accumulated during the reaction.…
Enzymes are biological catalysts. They speed up chemical reactions in all living things, and allow them to occur more effortlessly, without them we would not be alive. “Catalyst” denotes a substance that has the ability to increase the rate of a chemical reaction, and is not changed or destroyed by the chemical reaction that it accelerates (MicroTak, 2002). The enzyme Amylase speeds up the breakdown of starch into simple sugar; this reaction happens in the mouth and is the start of chemical digestion. Starch cannot pass through the lining of the intestine; it is too big (REFER TO FIGURE-31). The enzyme in question here is called Amylase; it catalyzes the hydrolysis of the polysaccharide starch (amylose) to the disaccharide maltose (Miller, 1992 ).…
Amylase is the type of enzyme that is used to convert starch into glucose so that it can be absorbed. Once the glucose has diffused into the blood stream it is carried to cells to be broken…
Introduction: This lab tested how enzymes are able to affect the rate of chemical reactions and how the rate of which an enzyme works in different conditions. The conditions the enzymes were tested in included…
The purpose of this lab experiment is to investigate factors that can affect the porcine pancreatic amylase enzyme activity in different environments such as the temperature, pH and also how being stored in extreme temperatures can affect the activity of the amylase. The activity of the amylase is going to be determined by the presence or absence of starch in the samples over time. There are some hypotheses on the Effects of temperature and pH; as I add the amylase to the starch in different temperatures the reaction’s rate increases in high temperatures; I belive that the amylase will work better. As the environment grows warmer, the amylase is going to become more energetic and more effective. Amylase is affected by environmental pH. I predict that the amylase activity will work best at a pH 7. As the pH changes from this point I predicted that the amylase activity is going to decrease and eventually stop. If I boiled and froze some amylase solution, and try to digest starch with at it at room temperature, I predict the previously-boiled and frozen amylase will not…
Enzymes are protein catalysts that speed up chemical reaction in living organisms. The purpose of this lab was to; observe the breakdown of hydrogen peroxide toxin by potato’s enzyme catalase, determine factors that influence how quickly the reaction takes place, and determine factors that influence how well enzymes function. The first test, we were supposed to see what would happen when we add sand in hydrogen peroxide. We filled a test tube with 5mL hydrogen peroxide and we added a pinch of sand into the test tube. For the first test tube we observed that the hydrogen peroxide did not react with the sand because there is no catalase in the sand. Our time for the reaction was zero seconds.…
Amylase is an enzyme in human saliva and in other organisms and its substrate is starch. When the active site of amylase binds with the starch, hydrolysis takes place. When the hydrolysis (the breaking of a chemical bond with the insertion of the ions of a water molecule) of starch is complete you are left with a disaccharide called maltose.…
To test the effect of a substrate concentration on enzyme activity, the amylase enzymes were combined with a different substrate concentration (starch) and the rate of the reaction was determined with the aid of I2kI. If starch was detected, the solution turned to dark blue; if the starch was already broken down, then reaction stayed colorless. To test the optimal PH, the starch and a buffer were combined at a specific PH level and the rate of reaction was tested. To determine the optimal temperature of amylase enzyme, the solution and amylases enzyme were held at various temperatures and the rate of reaction was determined.…
5. Amylase may be cleansing agent for oral cavity by digestion starch particles near or on teeth.…
Moreover, food is chemically digested by salivary amylase (carbohydrase) in saliva which is an enzyme (biological catalyst) which breaks down the insoluble polysaccharide starch to the soluble simpler sugar called maltose. In digestion, food molecules are broken down by hydrolysis reactions (breakdown with water). Saliva also contains mucus, which lubricates and helps hold together chewed food in a clump called a bolus. Hydrogencarbonate is also present in the mouth; this maintains an ideal pH for amylase to work in (pH6.5-7.5). Finally, as the tongue is muscular it can move food, so it therefore pushes food back to where it is swallowed.…