Review Sheet 39b

Topics: Enzyme, Digestion, Starch Pages: 4 (603 words) Published: August 29, 2011
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39 B

NAME ____________________________________ LAB TIME/DATE ________________________

Chemical and Physical Processes of Digestion: Computer Simulation

Carbohydrate Digestion
The following questions refer to Activity 1: Assessing Starch Digestion by Salivary Amylase. 1. At what pH did you see the highest activity of salivary amylase?



because that is when the salivary is most effective and it breaks down carbohydrates 2. How do you know that the amylase did not have any contaminating maltose?

because the sample didn't change color.

Negative sugar/IKI test. No change from original blue bright color.

We used a negative control to see if there is any amylase in the maltose 3. What effect did boiling have on enzyme activity? Why?

Boiling denatured the enzyme because it destroys the structure of the protein.

4. Describe the substrate and the subunit product of amylase. The substrate is starch the subunit is reducing sugar.

The following questions refer to Activity 2: Assessing Cellulose Digestion. 5. Does amylase use cellulose as a substrate? Explain.

No because amylase only works on starch and wont digest cellulose. 6. Did freezing have an effect on the activity of amylase? Explain. Freezing does not change the structure of the enzyme so it doesn’t have effect on the activity. 7. Do you think that the bacterial suspension contained the enzyme cellulase (an enzyme that digests cellulose)? Why or why not? Yes I think the bacteria contained cellulose because it specifically digests cellulose.

8. What is the substrate of peptidase? Explain, based upon your results. The substrate is a synthetic protein called APNA.



Review Sheet 39B

Protein Digestion by Pepsin
The following questions refer to Activity 3: Assessing Protein Digestion by Pepsin. 9. At which pH did you see the highest activity of pepsin? body?


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