Respiration in Peas Lab

Topics: Temperature, Carbon dioxide, Fahrenheit Pages: 8 (2191 words) Published: May 4, 2014

Cellular Respiration: Using the Vernier LabQuest to detect CO2 gas production in germinating peas

DESIGN

Research Question:
What is the effect of temperature on CO2 gas production in germinating peas?

Background: The process of cellular respiration, which provides energy for cell growth and development, is an important part of life in germinating seeds. It converts the chemical energy of glucose into ATP. As a germinating seed respires, CO2 is produced as a byproduct. Therefore, the amount of CO2 produced from a respiring seed can be an indicator of the amount of activity that is taking place in its cells. Germinating peas in particular undergo cellular respiration in their early stages of development, as they are often buried underground where there is no light present for photosynthesis to take place. For germinating peas, cellular respiration is a necessary component of growth and survival. The aim of this experiment is to investigate the effect of temperature of CO2 production in germinating peas.

Hypothesis: My hypothesis is that the germinating peas that are placed under a significantly cold temperature will, in general, produce less CO2 than the peas that are left at room temperature because this would denature the enzymes that are necessary for the cells of the peas to function. Secondly, I predict that CO2 production will increase with temperature until a certain point. Germinating peas have an optimal temperature for respiration. However, when the temperature increases significantly past optimal, it is possible that enzymes will denature, hindering or perhaps fully halting CO2 production.

Variables

Experimental Variables Units used to measure
Independent variable: temperatureDegrees Fahrenheit
Dependent variable: CO2 gas production in germinating peasPPM Relevant controlled variablesEffects of the variables on the outcome of the experiment if we ignored themPlan to control these variables Size of peasGerminating peas can vary in size which can affect total CO2 production Peas will be handpicked to be about the same size so that no significantly small or larger peas are used. Time peas have been germinatingPeas that have been germinating at different lengths of time can have an effect on CO2 production because as they grow, they begin to rely more on photosynthesis and less on respirationAll peas that are used will have been left to germinate for the same amount of time Wait time before Labquest begins collecting dataTime between equilibration and selecting ‘play’ on Labquest for data collection may vary. Data may be collected for different time periods in CO2 production.During the one-minute equilibration period, the Labquest will be set up so that data collection begins as close to the end of the equilibration period as possible.

Materials

-5 LabQuest data collection programs
-5 CO2 gas sensors
-5 250 mL respiration chambers (bottles)
-Paper towels
-125 germinating peas
-A few handfuls of glass beads
-Timer
-5 plastic tubs
-Ice cubes
-One water bath
-Thermometer measuring degrees Fahrenheit

Methods

1.A total of 125 germinating peas will be obtained, being wary of size. I will attempt to pick peas that are all of a similar size. 2.I will separate the germinating peas into 5 groups of 25 and mass one group using water displacement. Then I will obtain this same mass of glass beads and place these in one group, labeling each group 1 through 6. 3.Preparing the peas:

a.Cold peas:
i.One group of 25 germinating peas will be soaked in a plastic tub filled with ice water for 15 minutes 1.The temperature of the water will be recorded in degrees Fahrenheit in Table 1. ii.One group of 25 germinating peas will be soaked in cold tap water for 15 minutes 1.The temperature of the water will be recorded in degrees Fahrenheit in Table 1. b.Warm peas:

i.One group of 25 germinating peas will be soaked in a hot...

Cited: Banas, Timothy. "The Effect of Temperature on Pea Respiration." GardenGuides. N.p., n.d. Web. 17 Dec. 2013.
"Enzymes." Chemistry for Biologists:. N.p., n.d. Web. 16 Dec. 2013.
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