Renal Regulation of Blood Osmolarity

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Abstract: The experiment was done to demonstrate the effect of ADH on the volume and concentration of urine in order to demonstrate the control of ADH over blood plasma osmolarity. Since non-invasive methods were preferred the volume and concentration of urine was used in place of drawing blood. The results that we our anticipating are that ADH levels in the group of subjects that ingested the 6 gm. Of NaCl would increase over time in response to the increased osmolarity of the blood from all of the salt. Urine output would decrease and eventually the body would stabilize.

Purpose: In this experiment, renal regulation of osmolarity will be demonstrated through the use of urinalysis.

Materials and Methods: In this experiment, we assigned two groups. The first group was given 800ml of distilled to drink and the second was given 6mg of NaCl dissolved in a small amount of water. Both groups were instructed to note the time each time they voided from waking up until the control urine that was obtained just prior to the beginning of the experiment. They also were not to deviate from their normal activities of food and liquid consumption on this day. The control urine was the last urine prior to the experiment and was taken in a large specimen cup. The osmolarity of blood plasma is within normal range at 275-295 mosmol/L of blood. Due to the fact that urine is the end product of our filtered plasma, it was an appropriate and noninvasive vehicle to demonstrate how ADH secretion works in regulating osmolarity. The experiment began when the groups drank the entire solutions and the time was noted for that. Once the experiment was in progress, the groups were instructed not to ingest anything until the lab was complete. During the 30 minute waiting periods between voiding there were three tests to perform on the urine samples. The control urine was used for the first set of data and time was measured since last void. The total amount of time to



References: Kirkpatrick, W. Natriuretic Peptide Hormones. Anatomy & Physiology II, Spring 2010. Class Handout. Retrieved from: www.coursecompass.com/courses/1/kirkpatrick24127/content Martini, F., Ober, W., et al. Fundamentals of Anatomy & Physiology. Pearson Education Inc. 2006. 7th ed. www.microvet.arizona.edu/courses/vsc401/pdf_files/urinarysystem.PDF

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