The importance of identification of a certain microorganisms can range between a life threatening diseases to a creation of certain antibiotic. Understanding the principals of living microbes and identifying my unknown bacteria through numerous biochemical and metabolism tests, with the outmost confidence, Proteus vulgaris had the precise qualifications. The point of this report is to further explore the identification of my unknown bacteria by revealing the results of the experiments and comparing them to the other six known bacteria: Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Alcaligenes faecalis, Escherichia coli, and Proteus vulgaris that were used in the lab, as well as comparing and contrasting the actual and factual results.
Its unique pink rod shaped morphology was the first step observed under the microscope to identify its unknown characteristic. There were other methods utilized in lab as well: the Mannitol Salt and Eosin Methylene Blue Agar and the tryptic soy broth experiments. Oxygen reaction (aerobic vs. anaerobic), glucose fermentation, oxidase reaction, the catalase test, urea hydrolysis, nitrate reduction experimentation, Kligler’s Iron Agar, the SIM medium test and lastly the IMViC series of tests. All the biochemical tests were carried out in properly supervised manner to compare the unknown bacteria to the six known that were mentioned above.
First it was the Gram staining procedure used to categorize bacteria in two groups, gram positive and gram negative. Gram staining is one of the most useful test used in the clinical setting to identify bacterial colonies due to their broad staining spectrum. (Black, 2008, pp. 70-71) The basis of the Gram stain is that gram positive bacteria retains the color of the primary dye, the crystal violet, whereas the gram negative bacteria loses the primary dye once its washed with a decolorizing solution but takes on the color of the counterstaining dye of
References: BD™ Tryptic Soy Broth (TSB). (2008, February). Retrieved from Becton, Dickinson and Company: http://www.bd.com/europe/regulatory/Assets/IFU/HB/CE/BA/BA-257107.pdf Black, J. G. (2008). Microbiology Principles and Explorations (Vol. 7th Edition). Hoboken, NJ: John Wiley & Sons, Inc. Fred D. Williams and Robert Schwarzhoff. (October 1978). Nature of Swarming Phenomenon in Proteus. Annual Review of Microbiology , 37. Harley, J. P. (2011). Laboratory Exercises in Microbiology (Eighth Edition ed.). New York, NY: McGraw- Hill. Robert S. Breed, E.G.D. Murray, Nathan R. Smith. (1957). Bergey 's Manual of Determinative Bacteriology (Seven ed.). Baltimore: The Williams & Wilkins Company.