Protein Synthesis Lab
Laboratory Exercise #3
Measuring Protein in Solution
Abstract
The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also, to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so, egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added, the amount of protein could then be determined. After that, 14 test tubes were used to create a set of protein standards. Biuret solution was added to all 22 tubes and vortexed. Absorbance data was then collected by these protein standards using the SpectroVis Plus, and there was a direct relationship between absorbance …show more content…
However, there was a typo in the lab on page 39, step number 10. It says to add 0.666uL of Biuret solution to all 22 test tubes, but it was supposed to say 0.666mL. Therefore, the correct results were provided to the class because the data the class recorded was done will 0.666uL of Biuret solution. …show more content…
As clearly shown by the tables and the graph, the Biuret was useful in doing so- not only for known solutions, but also unknown solutions. In order to determine the concentration of protein in an unknown solution, the graph was used to see where the absorbance fell on the standard curve. In this lab, there was a typo in the lab, resulting in inaccurate results by the entire class. Therefore, the proper typical results of this experiment were provided by the instructor in order to get a better representation of the data in the experiment to be used for the standard curve. The Biuret assay is a very useful tool by scientists, particularly because it is used to determine the amount of protein in foods and drinks on the market, which is necessary because it is required by law to state the nutrition facts on the labels of all food products. The Biuret assay was also useful in the experiment done by P. Zhou and J.M. Regenstein, who used it to determine the concentration of protein in cold water fish gelatins as well as warm water fish, avian, and mammalian
Measuring Protein in Solution
Abstract
The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also, to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so, egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added, the amount of protein could then be determined. After that, 14 test tubes were used to create a set of protein standards. Biuret solution was added to all 22 tubes and vortexed. Absorbance data was then collected by these protein standards using the SpectroVis Plus, and there was a direct relationship between absorbance …show more content…
However, there was a typo in the lab on page 39, step number 10. It says to add 0.666uL of Biuret solution to all 22 test tubes, but it was supposed to say 0.666mL. Therefore, the correct results were provided to the class because the data the class recorded was done will 0.666uL of Biuret solution. …show more content…
As clearly shown by the tables and the graph, the Biuret was useful in doing so- not only for known solutions, but also unknown solutions. In order to determine the concentration of protein in an unknown solution, the graph was used to see where the absorbance fell on the standard curve. In this lab, there was a typo in the lab, resulting in inaccurate results by the entire class. Therefore, the proper typical results of this experiment were provided by the instructor in order to get a better representation of the data in the experiment to be used for the standard curve. The Biuret assay is a very useful tool by scientists, particularly because it is used to determine the amount of protein in foods and drinks on the market, which is necessary because it is required by law to state the nutrition facts on the labels of all food products. The Biuret assay was also useful in the experiment done by P. Zhou and J.M. Regenstein, who used it to determine the concentration of protein in cold water fish gelatins as well as warm water fish, avian, and mammalian