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Pros And Cons Of Optimal Media

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Pros And Cons Of Optimal Media
Five plates with minimal media were divided into quadrants. Each plate was also marked with the numbers 90, 262, 66, 163, and 491 to correspond with which mutant was placed inside. Additionally, the plates were marked with ARG, CIT, PRO, and ORN in each quadrant to correspond with the supplements of arginine, citrulline, proline, and ornithine. In addition, a sixth plate was divided into halves for the control group, 262. This plate was marked with ARG and PRO on one half and nothing was marked on the other. Next, about 1.0 ml of saline solution was added to each culture. It was mixed to form a spore suspension. Then six drops of each spore suspension were added to the soft agar that is stored at 45-47C. This was then poured on the minimal
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