The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this, a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next, an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B, bovine serum albumin, ovalbumin, and carbonic anhydrase) will be placed into separate wells within the gel, and the apparatus will be connected to a constant voltage source (175 V) for an allotted period of time (60 min). This will allow electricity to flow through the apparatus,…
Consider the pathway for the synthesis of the amino acid arginine in Neurospora: ARG-E ARG-F ARG-H ornithine → citrulline → argininosuccinate → arginine Mutant strains of Neurospora are grown in minimal media supplements as follows. Strains may carry more than one mutation. Growth is shown by (+) no growth is shown by (o). Supplements mutant nothing ornitihine citrulline argininoarginine strain succinate a o o o o + Indicate the most accurate statement regarding strain A. A) There is a mutation in ARG-H, if citrulline accumulates, ARG-F is also defective. B) There is a mutation in ARG-H, if ornithine accumulates, ARG-F is also defective. C) There is a mutation in ARG-H, if argininosuccinate accumulates, ARG-F is also defective. D) There is a mutation in ARG-H, if citrulline accumulates, ARG-E is also defective. E) There is a mutation in ARG-E, if citrulline accumulates, ARG-F is also defective. Ans: A 23. Shown below are the deletion maps of a series of rII- mutations. The deleted region is indicated as (......) and the intact region as ______. 1 ___________(...........)_______________ 2 _________________(...........)_________ 3 (.....................)_______________ ______ 4 ________________________(................) 5 _____(..........)______________________ A series of point mutations A-E is used in a coinfection experiment. Shown below are the results of those coinfections. Ability to produce wild-type progeny phage is indicated by (+), (o)…
If a growing colony is dark under UV light, a mutation has occurred. Methods E. coli mutator was transformed with pGLO. A broth was inoculated and serial passaging was performed for seven days. A kit was then used for DNA purification. Competent cells were mixed with the DNA samples in an ice bath.…
(mutants) can only grow in a minimal medium that is supplemented with specific intermediates found in the…
The variables were kept controlled by using different inoculating loops to add and spread out each solution. This was done to prevent any solutions from mixing together. The same amount of pGLO plasmid solution was added by dipping the loop until a bubble was formed across the loop opening. The tubes were incubated for the same amount of time and heat shocked for the same amount of time. The same amounts of Luria broth and cell suspension were used to keep the number of individual colonies controlled.…
Rebecca Ann Lind in “Considerations of Media Effects” in Race/Gender/Media: Considering Diversity Across Audiences, Content and Producers discusses the social psychology of stereotypes, cultivation theory, global media influences, and formation of self-image. First off, schemas describe expectations one has about the world. Stereotype, a type of schema, shows how one distinguish knowledge on social groups. The cultivation theory expresses that viewers construct a perception of the world through what they see on television. Television cultivates ideas about marriage, interpersonal relationships, family, and beauty. Likewise, television programs from the United States have the power to globally influence those in different cultures and create an ideological homogenization of the world. Lastly, the levels of television consumption affect individuals and the creations of their self-image.…
2 Frankel, J., et al. Principles of Biology I Laboratory Manual Fifth Edition. Pearson Custom Publishing, Boston. 2005. pp.37-42…
Convenient and Flexible: The television is a convenient and flexible advertising medium, owing to its widespread popularity and the ease with which a message can reach millions of viewers internationally and nationally. Television advertising allows advertisers the flexibility to use various approaches and different combinations of audio, video and text to make ads memorable and emotional, depending on the product or service or the target audience.…
Your question is quite vague, so I'm not sure what you're really looking for here.…
3 YPD media agar plates were obtained and labeled with group number and the date. Each plate was also labeled 0.1 mL, 0.2 mL, 0.4 mL, these corresponded to the volume of mutated cell culture…
The different types of media include print media, electronic media and new age media. Print media includes newspapers, books and pamphlets. Electronic media includes television and radio while the new age media include the internet and mobile phones.…
Media envelops our homes, our culture and our world. Books, newspaper, magazines, radios, television, movies and computers are examples of media, which have encompassed us throughout the ages. Whether being bombarded by these facets of media is good for us or not, we must face the reality if its presence in our lives. However, how should we allow it to affect us?…
For the bacteria’s case, using a sterile borer, 5 mm diameter wells are mixed into the medium. The samples are inoculated with the test bacterium which needs to be adjusted to the 0.5 McFarland standard solution; a sterile cotton swab was dipped into the suspension, rotated several times, and pressed firmly on the inside wall of the tube above the fluid level removing excess inoculum. The surface of the agar plate was streaked over the entire sterile agar surface rotating the plate to ensure an even distribution of inoculum with a final swab around the rim. The plates need to dry for 3-5 min. Fifty uL aliquots of each test extract are dispensed into each well after the inoculation of the plates with bacteria. The wells are arranged 2 inches apart in a triangular form. Three plates are used for the extracting of bacterium and the same extract is used for each plate. For each bacterial strain, controls are maintained where pure solvents are used instead of the extract. The plates are sealed with parafilm, labeled, and placed in an incubator set to 37°C. After 24 hours of incubation, each plate should be examined for inhibition zones by using a ruler in millimeters (Biswas & et al,…
Means of media are the instruments or devices to disseminate information to common people. Media can be used for promotions of products and services, For instance, news paper, TVs, advertisements, radio announcements can be used as a means of communication. These are the form of entertainment. These are the means in which globalization takes place, a means of informing people about events from local to international to global miscommunications.…
As the number of Internet users continues to grow, do you think that the Internet will change the way that we read and think about the news? As more and more people, especially young people, turn to the Internet for news about world and local events, we find that newspapers, television, and radio news have been losing their audience or even going out of business. Some people are concerned about this shift because newspapers provide important in-depth coverage of events, while some Internet news sites only report opinions or repeat what is already in the newspapers. However, the new media offer so many advantages over the traditional media that they could easily become better sources of news than any we have ever had.…