Pp Lab Report Digestion

LABORATORY REPORT
Activity:

Effect of Dietary Fiber on Transit Time and Bile

Name:

Hope Schallert

Instructor:

Dr. Weithop

Date:

03.08.2015

Predictions
Dietary fiber will: increase transit time
Dietary fiber will increase the amount of bile acid in solution

Materials and Methods
Effect of Dietary Fiber on Transit Time
1. Dependent variable. number of radiopaque markers in GI tract
2. Independent variable. amount and type of fiber in meal
3. Controlled variables. calories in meal, nutrients in meal

Effect of Dietary Fiber on Bile Acid Adsorption
1. Dependent variable. amount of bile acids in solution
2. Independent variable. amount of fiber in meal
3. Controlled variables. moisture in meal, size of meal particles, calories and nutrients in meal
4. Name the type of method used to measure transit time.
Radiopaque
5. Name the type of method used to measure the amount of bile acids in solution.
Spectrophotometer

Results
Table 3. Effect of Fiber on Transit Time - Number of Markers in Stomach

Subject 1
Subject 2
Subject 3
Average
Average percentage of the total markers Standard diet
Minutes after markers ingestion.
Number of markers present.
60 min
180 min
240 min
18
7
0
19
6
0
19
5
0
19
6
0
79
25
0

Insoluble fiber-rich diet
Minutes after markers ingestion.
Number of markers present.
60 min
180 min
240 min
21
6
1
19
7
0
18
8
1
19
7
1
79
29
4

Soluble fiber-rich diet
Minutes after markers ingestion.
Number of markers present.
60 min
180 min
240 min
20
10
1
19
9
3
20
8
2
20
9
2
83
38
8

Laboratory Report/ Hope Schallert/ Effect of Dietary Fiber on Transit Time and Bile/ Dr. Weithop / 03.08.2015/ Page [1] of [4]

Food Transition Through the Stomach

1. For the standard meal, what is the average percentage of markers remaining in the stomach at 60 min, 180 min, and 240 min?
79, 25, 0
2. For the meal with insoluble fibers, what is the average percentage of markers remaining in the stomach at 60 min, 180 min, and 240 minutes? 79, 29, 4
2. For the meal with