The purpose of this experiment is to calculate the mass/change in mass of plant tissue, in our case potato tubers. The change in mass of the potato tuber disk determines the osmotic concentration. After soaking the potato tubers in different concentrations of sucrose, then calculating the percent change in mass, we can determine the osmotic concentration. The…
The first experiment with 1 M sucrose in the bag and distilled water in the beaker had almost exactly the same results. The masses were relatively the same the difference have could been from the amount of liquid that was placed inside of each of the bags. The second experiment with 5% Ovalbumin in the bag and 1 M sucrose in the beaker had almost exactly the same results. The masses were relatively the same the difference could have been from the amount of liquid that was placed inside the bag.…
Since the change in mass of the potato tubers was negative in the sucrose solutions from 0.2 mM to 0.5 mM, these solutions were hypertonic relative to the potato dicks. In the solution with only 0.1 mM of sucrose, the mass of the potato tubers increased which means that this…
As the concentration of the sucrose solution increases, the average percentage change in mass decreases in the potato tubers and this is the same as in the carrot tubers. At low concentrations of sucrose solutions (0.1 M) the mass of the carrot and potato tubers increases due to water moving into the protoplast of the cell from the sucrose solution by osmosis and at high concentrations of sucrose solutions (0.5 M) the mass of the carrot and potato tubers decreases due to water moving out of the protoplast of the cell to the sucrose solution by osmosis. At certain concentrations (0.18 M of the potato and 0.355 M for the carrot) the potato and carrot tubers don’t change in mass due to the water potential inside the cells equalling the water potential of the sucrose solution. My graph displays a distinct negative correlation; the higher the concentration of sucrose…
1. Some potatoes increased in mass while others decreased due to osmosis. Potatoes that had a higher concentration increased in mass because water passed through the membrane into the potato in an effort to dilute it. Potatoes that had a lower concentration than the solution decreased in mass because water flowed through the membrane into the solution trying to dilute the solution. The water moves to the higher concentration to dilute it/maintain homeostasis, increasing or decreasing mass depending on whether it goes in or out.…
Enzymes are catalysts that speed up chemical reaction but are not themselves consumed or changed by the reaction. The cell’s biological catalysts are proteins. Proteins are made up of one or more polypeptide chains that are folded to make an active site, an area in which a material to be acted on by the enzyme, called the substrate, will fit. The temperature,pH, the concentration of enzyme, and the concentration of substrate all affect the activity of the enzyme and the rate of the reaction.…
This experiment gave a visual understanding of osmosis and diffusion. The first experiment proved that solutes would move down a concentration gradient if permeable to the selective membrane. The second experiment proved different solute concentrations affect the movement of water, depending on the solute concentration inside the cell. The purpose of this lab was to look for different solutes that can cross an artificial membrane and to observe the effect of different concentrations of sucrose on the mass of a potato cell. Results for Part One suggested that the molecular weight of albumin and starch was too large to pass through the dialysis tube, but glucose and sodium sulfate molecules were small enough to pass through the dialysis tube. Also, a decrease in water weight occurred due the dialysis tube being placed in a hypertonic solution. Results for Par Two showed the potato cell having a molar concentration of 0.2734, which caused sucrose concentrations above 0.2 M to have a decrease in mass. Inversely, sucrose concentrations below 0.2 M caused an increase in mass.…
As the concentration of a solute in water will, through osmosis, transfer until the concentration is nearly the same both inside the cell, or in this case potato, and outside of the cell, the potato strips both gained and lost mass due to the different concentrations of sugar in water. As expected, the potato strips placed in the concentration of sugar water closest to the concentration of sugar water in a potato had the least amount of change in mass. As the potato strips placed in substance Z and substance A both changed 7%, the concentration of sugar water in a potato must be somewhere in between a concentration of 0.4 and 0.6. One source of error for this lab is that the amount of substance placed into each Dixie cup was not measured and therefore were not consistent. If this lab were repeated, it is suggested that the amount of substance be measured in order to produce more consistent results.…
My hypothesis in this experiment was that a Yukon gold potato will naturally absorb more water than a sweet potato due to the effects of osmosis. Osmosis plays a huge role in biology for a multitude of reasons. As defined by Freeman (2012) “The movement of water is a special case of diffusion that is given its own name: osmosis, occurs only when solutions are separated by a membrane that is permeable to some molecules but not others- that is, a selectively permeable membrane.”(P.90). It is also a type of passive transport, as it does not require energy.…
Through osmosis, I hypothesise that as the solution of the starch in each tube increases, the water content being reduced will cause the potato to release more water through the membrane to balance the starch solution, resulting in a decrease in the mass of the potatoes as the respective starch concentration increases, though maybe not for the 0% solution. Risk Assessment The potential danger of this experiment is relatively low. Nevertheless, lab coats and eye protection were worn when handling the starch concentration and extreme care was taken when cutting and weighing the potato using a scalpel on a slip-proof…
Conclusion: My hypothesis was correct; the potato cubes placed in distilled water and 0.2 M sucrose solution gained weight, starting with 5.97 grams and 7.35 grams respectively and ending with _____g and _____g. The ones in 1 M and 0.8 M…
Enzymes are protein catalysts that speed up chemical reaction in living organisms. The purpose of this lab was to; observe the breakdown of hydrogen peroxide toxin by potato’s enzyme catalase, determine factors that influence how quickly the reaction takes place, and determine factors that influence how well enzymes function. The first test, we were supposed to see what would happen when we add sand in hydrogen peroxide. We filled a test tube with 5mL hydrogen peroxide and we added a pinch of sand into the test tube. For the first test tube we observed that the hydrogen peroxide did not react with the sand because there is no catalase in the sand. Our time for the reaction was zero seconds.…
This overall flow of water from a dilute area of high water potential to a more concentrated solution of low water potential across a partially permeable membrane is called osmosis. I predicted that the swede cylinders which are put in a test tube with a low potential of sucrose solution would become turgid because the water molecules that are present in the swede will move away from an area of higher potential of water molecules to an area that has a lower potential of water molecules, this means that the swede sample will gain mass and become full almost to an extent where it is ready to burst. The swede samples that are going to be put in a test tube with a high potential of sucrose solution will become flaccid because the swede cylinder will have a higher potential of water molecules and so these molecules will diffuse into the sucrose solution as it has a lower water potential, this means that the potato sample will shrivel and loose mass.…
The results seen in the water control as well as possibly the 0.2M sucrose occur because the water potential outside of the potatoes would be higher thus encouraging water to move into the potatoes and increase their mass. In other words, osmosis (diffusion of water from high to low concentration across a membrane) occurred and moved water from the environment hypotonic to the carrot into the carrot itself in an attempt to equalize concentration between them. In the higher molarity sucrose solutions, water’s potential is higher inside the potatoes—the water content of the carrots is more pure than in the outside solutions. Thus water would move out of the carrots into the…
Then you have our standard for have a standard for the rest of the experiment.…