Potato Catalase Lab Report
I. Experiment 1
A. Description of Experiment: Three plastic beakers were used for this experiment. In one, a 20 mL of 1% hydrogen peroxide solution was made. In order to get this, the stock solution, which was 3%, was diluted using 10 mL of hydrogen peroxide and 20 mL of water. In the second beaker, a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay was performed. Using forceps a small paper disk was dipped into the second beaker containing the potato catalase for exactly 5 seconds. It was next dried on a paper towel for 5 seconds, and finally placed at the bottom of the first beaker, which contained hydrogen peroxide solution. The timer was then started. The timer stopped when the paper disk floated up to the top of the beaker. This was performed 3 times. After this, two negative controls were tested. The first negative control used a denatured catalase instead of the potato extract. The …show more content…
Five beakers were used for this lab. The first beaker was labeled catalase and contained a 1/4 dilution of the catalase solution, (2 mL of catalase and 6 mL of water). The second beaker was labeled room and contained a 1/3 dilution of H2O2, 10 mL of H2O2 and 20 mL of water. This beaker was left on the table in room temperature. The third beaker was labeled 37° and contained 1/3 dilution of H2O2, same as above. This beaker was placed in in a water bath set to 37° c to equilbriate. The fourth beaker was labeled 59° c and contained 1/3 dilution of H2O2. This beaker was placed in a water bath set to 59° c to equlibriate. The fifth beaker was labeled cold and contained a 1/3 dilution of H2O2. This beaker was placed into a cup of ice to equilibrate. The beakers equilibrated for about 15 minutes. The assay fwas performed three times for each of the different H2O2 dilution temperatures. Data was measure and
A. Description of Experiment: Three plastic beakers were used for this experiment. In one, a 20 mL of 1% hydrogen peroxide solution was made. In order to get this, the stock solution, which was 3%, was diluted using 10 mL of hydrogen peroxide and 20 mL of water. In the second beaker, a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay was performed. Using forceps a small paper disk was dipped into the second beaker containing the potato catalase for exactly 5 seconds. It was next dried on a paper towel for 5 seconds, and finally placed at the bottom of the first beaker, which contained hydrogen peroxide solution. The timer was then started. The timer stopped when the paper disk floated up to the top of the beaker. This was performed 3 times. After this, two negative controls were tested. The first negative control used a denatured catalase instead of the potato extract. The …show more content…
Five beakers were used for this lab. The first beaker was labeled catalase and contained a 1/4 dilution of the catalase solution, (2 mL of catalase and 6 mL of water). The second beaker was labeled room and contained a 1/3 dilution of H2O2, 10 mL of H2O2 and 20 mL of water. This beaker was left on the table in room temperature. The third beaker was labeled 37° and contained 1/3 dilution of H2O2, same as above. This beaker was placed in in a water bath set to 37° c to equilbriate. The fourth beaker was labeled 59° c and contained 1/3 dilution of H2O2. This beaker was placed in a water bath set to 59° c to equlibriate. The fifth beaker was labeled cold and contained a 1/3 dilution of H2O2. This beaker was placed into a cup of ice to equilibrate. The beakers equilibrated for about 15 minutes. The assay fwas performed three times for each of the different H2O2 dilution temperatures. Data was measure and