Plant Tissue Culture Techniques
Plant Tissue Culture
151
Chapter 9 Plant Tissue Culture Techniques
Lorraine Mineo
Department of Biology Lafayette College Easton, Pennsylvania 18042
Lorraine Buzas Mineo (B.S., Muhlenberg College; M.A., Duke University) is a lecturer in the Department of Biology, Lafayette College, and has taught botany since 1978 and supervised the General Biology Laboratories since 1970. Research interests in physiological and forest ecology have culminated in several publications. Other interests include science education methods.
Reprinted from: Mineo, L. 1990. Plant tissue culture techniques. Pages 151-174, in Tested studies for laboratory teaching. Volume 11. (C. A. Goldman, Editor). Proceedings of the Eleventh Workshop/Conference of the Association for Biology Laboratory Education (ABLE), 195 pages. - Copyright policy: http://www.zoo.utoronto.ca/able/volumes/copyright.htm Although the laboratory exercises in ABLE proceedings volumes have been tested and due consideration has been given to safety, individuals performing these exercises must assume all responsibility for risk. The Association for Biology Laboratory Education (ABLE) disclaims any liability with regards to safety in connection with the use of the exercises in its proceedings volumes.
© 1990 Lorraine Mineo 151
Association for Biology Laboratory Education (ABLE) ~ http://www.zoo.utoronto.ca/able
152
Plant Tissue Culture
Contents Introduction....................................................................................................................152 Terminology...................................................................................................................152 Laboratory Requirements for Tissue Culture ................................................................153 Demonstration of "in vitro" Morphogenesis and Totipotency of Seedling Explants ....154 Effects of Hormone Balance on Explant Growth and Morphogenesis..........................160 Callus Formation
151
Chapter 9 Plant Tissue Culture Techniques
Lorraine Mineo
Department of Biology Lafayette College Easton, Pennsylvania 18042
Lorraine Buzas Mineo (B.S., Muhlenberg College; M.A., Duke University) is a lecturer in the Department of Biology, Lafayette College, and has taught botany since 1978 and supervised the General Biology Laboratories since 1970. Research interests in physiological and forest ecology have culminated in several publications. Other interests include science education methods.
Reprinted from: Mineo, L. 1990. Plant tissue culture techniques. Pages 151-174, in Tested studies for laboratory teaching. Volume 11. (C. A. Goldman, Editor). Proceedings of the Eleventh Workshop/Conference of the Association for Biology Laboratory Education (ABLE), 195 pages. - Copyright policy: http://www.zoo.utoronto.ca/able/volumes/copyright.htm Although the laboratory exercises in ABLE proceedings volumes have been tested and due consideration has been given to safety, individuals performing these exercises must assume all responsibility for risk. The Association for Biology Laboratory Education (ABLE) disclaims any liability with regards to safety in connection with the use of the exercises in its proceedings volumes.
© 1990 Lorraine Mineo 151
Association for Biology Laboratory Education (ABLE) ~ http://www.zoo.utoronto.ca/able
152
Plant Tissue Culture
Contents Introduction....................................................................................................................152 Terminology...................................................................................................................152 Laboratory Requirements for Tissue Culture ................................................................153 Demonstration of "in vitro" Morphogenesis and Totipotency of Seedling Explants ....154 Effects of Hormone Balance on Explant Growth and Morphogenesis..........................160 Callus Formation