Preview

Physioex 8.0 Experiment 1

Good Essays
Open Document
Open Document
1188 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Physioex 8.0 Experiment 1
Lab Report One

Exercise Five

Activity One
“Simulating Simple Diffusion”

Activity Two
“Simulating Facilitated Diffusion”

Jessica Ogola

Anatomy and Physiology 2401
Dr. Denyse Jones
02/02/2012

Objective:
The objective of this experiment is to perform the simulation of the movement of solutes from a higher concentration to a lower concentration within a given amount of time.

The goal of the exercise is to provide a simulation for the process of the facilitated diffusion of glucose through a selectively permeable membrane.

Introduction:
Experiment #1 and #2 are meant to show the processes of Simple and Facilitated diffusion. Both the solutes and water were used to show how they diffuse through a semi permeable membrane. In experiment #1 the process of using the solute and deionized water shows how much of the solute is passed through the membrane. In experiment #2 the rate of facilitated diffusion relied on the amount of carrier proteins available to transport the solute molecules.

Hypothesis – Activity 1: Simulating Simple Diffusion
“I hypothesize that all of the solutes will have some rate of diffusion through the permeable membrane.” Hypothesis – Activity 2: Simulating Facilitated Diffusion
“I hypothesize that a higher concentration gradient would result in a higher rate of glucose transport”

Materials and Methods:
Activity #1 Materials: * 2 Beakers * 4 MWCO Membranes * 20, 50, 100, 200 * 9.00 mM of each solute: * NaCl, Urea, Albumin, Glucose * Deionized water
Methods:
* The solutes NaCl, Urea, Albumin, and Glucose each in the amount of 9.00 mM were used respectively. * The first step is to place the appropriate membrane in between the beakers. * Dispense the appropriate solute (L) and Deionized water (R) into the beakers. * Set a timer for sixty minutes. When the time is finished read and record the data. * These steps are repeated for each solute and each additional



References: Human Anatomy and Physiology Laboratory Manual by Elaine N. Marieb and Susan Mitchell PhysioEx 8.0 Laboratory Experiments in Physiology http://www.biology-questions-and-answers.com/cell-membrane.html

You May Also Find These Documents Helpful

  • Good Essays

    3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction?…

    • 1043 Words
    • 5 Pages
    Good Essays
  • Good Essays

    3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction?…

    • 1338 Words
    • 6 Pages
    Good Essays
  • Powerful Essays

    To determine which way substances will move passively through a dif- ferentially permeable membrane (given the appropriate information on concentration differences). 01_001_016_PhyEx8_AP_Ch01 1/10/08 5:27 PM Page 1 The diffusion of solute particles dissolved in water through a differentially permeable membrane is called simple diffu- sion. The diffusion of water through a differentially perme- able membrane is called osmosis.…

    • 4065 Words
    • 27 Pages
    Powerful Essays
  • Good Essays

    Rubber Egg Lab

    • 893 Words
    • 3 Pages

    2. Explain your observations in detail in terms of concentration gradient, diffusion, osmosis, osmotic pressure, passive transport, and active transport.…

    • 893 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Lab 3 - Cells

    • 869 Words
    • 4 Pages

    Explain one way in which facilitated diffusion is the same as simple diffusion and one way in which it differs.__ Both are passive diffusion that are from high concentration gradient to a low concentration gradient. Facilitated differs from simple because it requires a carrier protein to diffuse. _…

    • 869 Words
    • 4 Pages
    Satisfactory Essays
  • Good Essays

    Diffusion and Glucose

    • 1471 Words
    • 6 Pages

    The larger value obtained when more glucose carriers were present corresponds to an increase in the rate of glucose transport. Explain why the rate increased. How well did the results compare with your prediction? The greater the concentration difference between one side of the membrane and the other, the greater the rate of facilitated diffusion.…

    • 1471 Words
    • 6 Pages
    Good Essays
  • Satisfactory Essays

    PhysioEx Exercise 1

    • 435 Words
    • 2 Pages

    Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report…

    • 435 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    The purpose of this study is to be able to identify what molecules can pass through a semi-permeable membrane and also to differentiate between diffusion and osmosis.…

    • 510 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Exercise 1

    • 694 Words
    • 3 Pages

    4. Explain why the Na+ and K+ transports were inaffected by the addition of glucose.…

    • 694 Words
    • 3 Pages
    Powerful Essays
  • Good Essays

    Ap Bio Lab One

    • 432 Words
    • 2 Pages

    This lab helps further our understanding of essential principles behind diffusion and osmosis. Permeability, concentration gradients, plasmolysis, water potential, and equilibrium were also concepts that were delved into in this lab. Understanding how diffusion and osmosis works is essential to understanding biology. Each time a cell has something move into or out of it, some sort of principle studied in this lab is occurring. Diffusion, osmosis, and passive and active transport are all fundamental concepts of Biology. This lab simulated osmosis in the cell. In this case, we used dialysis tubing to simulate the semi-permeable membrane of an animal cell.…

    • 432 Words
    • 2 Pages
    Good Essays
  • Satisfactory Essays

    Experimentation

    • 604 Words
    • 18 Pages

    Experiment Results Predict Question: Predict Question 1: What effect do you think increasing the number of protein carriers will have on the glucose transport rate? Your answer : a. The glucose transport rate will decrease. Predict Question 2: What effect do you think adding Na+ Cl- will have on the glucose transport rate? Your answer : c. There will be no change in the glucose transport rate. Stop & Think Questions: What happened to the glucose transport rate when the glucose concentration was increased from 2 mM to 8 mM? Your answer : c. The glucose transport rate was unchanged. Correct answer: a. The glucose transport rate increased. Describe the conditions that resulted in the fastest rate of glucose transport. You correctly answered: d. 700 glucose carrier proteins and 8 mM glucose Why do you think equilibrium wasn't reached? You correctly answered: b. The membrane proteins were saturated. Experiment Data: Run Number 1 2 3 4 5 6 6 Solute Glucose Glucose Glucose Glucose Glucose Na+ ClGlucose Start Conc. L 2.00 8.00 2.00 8.00 10.00 0.00 2.00 Start Conc. R 0.00 0.00 0.00 0.00 0.00 2.00 0.00 Carriers 500 500 700 700 100 700 700 Rate 0.0008 0.0023 0.0010 0.0031 0.0017# 0.0042 0.0010…

    • 604 Words
    • 18 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Facilitated diffusion depends on passive transport. Molecules cross cell membrane by diffusion. A type of diffusion is osmosis. If no energy is input into the system during diffusion, the molecules will reach a state of equilibrium. The purpose of this experiment was to study why the death occurred among those who drank salt water.…

    • 291 Words
    • 2 Pages
    Satisfactory Essays
  • Powerful Essays

    Bibliography: Bailey, R. (2013) Diffusion and Passive Transport. Available at: http://biology.about.com/od/cellularprocesses/ss/diffusion_2.htm (Accessed 5th March 2013).…

    • 1729 Words
    • 7 Pages
    Powerful Essays
  • Good Essays

    Review Sheet 1

    • 667 Words
    • 3 Pages

    Using your results from Chart 2, what was the fastest rate of facilitated diffusion recorded?…

    • 667 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Activties 1 Assigment 4

    • 200 Words
    • 1 Page

    3. Why did increasing the pressure increase the filtration rate but not the concentration of solutes? How well did the results compare with your prediction?…

    • 200 Words
    • 1 Page
    Satisfactory Essays