Intro to Biology
September 26, 2011 Lab Partners: Kristen, Tania and Betty Introduction
When using different methods to measure pH levels there are some tools that can be useful. Some more than others but by putting into action the different methods it may determine which tools will work best and give the best results when testing the pH within a solution. The pH, which stands for the proportion of hydrogen ions in a solution, could be acidic (acidosis), neutral or basic (alkaline). The pH scale goes from numbers 1 through 14. A pH of 7 is neutral; meaning the amount of hydrogen ions and hydroxide ions is equal, just like water.
An acid is an ionic compound that gives off hydrogen ions into a solution. An ionic compound is the result of an ionic bond, which are formed after atoms transfer electrons to achieve full outermost energy levels or orbits. Bases are ionic compounds that give off hydroxide into a solution or accept hydrogen. Buffers are what keep them at bay by using weak acids to maintain the pH levels. By using a pH paper, indicator dyes and a pH meter, several tests will be conducted to check which one will result in a precise pH level reading.
Materials and Methods
Get 5 beakers and label them A through E. Fill the beakers with 20 to 25 milliliters of the appropriate solutions and then cut a piece of pH paper at least one inch in length. Dip the pH paper into the solution and color coordinate with the pH chart it provides. Using indicator dyes. Get 2 sets of test tubes and the label them A through E. Fill the tubes with equal amounts of solution and then in only the first set of tubes, place 2 drops of Bromothymol Blue dye into each and make sure it mixes in well with the solutions. On the second set of tubes do the same but this time
References: Enger, D. E & Ross, C. F & Bailey, B. D (2011). Acids, Bases and Salts. Concepts of Biology. 14th Edition. Pg.34. Siegfried, R. D & Kratz, F. R (2010). “Ph”iguring out the pH scale. Biology for Dummies. 2ndEdition. Pg.42. ****Need help with citation of book reference and lab report.*********