Pglo Transformation Lab Report
Genetic transformation happens when an organism is altered by the introduction of new genetic information which is merged into the organism’s genome. Bacterial transformation is a type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab, the engineered pGLO plasmid is integrated into E. Coli bacteria, and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan, Studies on transformation of Escherichia coli with plasmids, 1983). To see the reaction of this plasmid on the cells, bacteria treated with the plasmid were grown on two separate agar plates containing LB nutrient broth and ampicillin, and another containing LB nutrient broth, ampicillin
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It is when a bacterial cell takes up DNA that is foreign to it and integrates it into its own DNA. What is genetic transformation? That is change that is caused by genes involving the insertion of a gene into an organism to change the organism trait. This transformation typically occurs in plasmids. So, what are plasmids? Plasmids are small circular DNA molecules that are isolated from its chromosomes. So, what are genes and plasmids and how are they related to bacteria and genetic transformation. (Hanahan, Jessee, & Bloom, Plasmid transformation of Escherichia coli and other bacteria, 1991)Well genes are part of DNA which basically give instructions to make molecules which are called proteins. In this bacteria transformation procedure bacteria are transformed with a gene that has codes for GFP (Green Fluorescent Protein). The bacteria with this gene will cause them to glow a brilliant green color under UV light. So, plasmids carry a gene that contains one or more traits that may be beneficial to survival. In this scenario, the pGLO has a resistance to antibiotics such as ampicillins, which is the one used in this method along with the bacteria E coli because it can easily be grown gene can be turned on in transformed cells by adding arabinose to the cells. SO therefore, the transformed cells will grow on plates with LB/amp and appear white on plates not contain
It is when a bacterial cell takes up DNA that is foreign to it and integrates it into its own DNA. What is genetic transformation? That is change that is caused by genes involving the insertion of a gene into an organism to change the organism trait. This transformation typically occurs in plasmids. So, what are plasmids? Plasmids are small circular DNA molecules that are isolated from its chromosomes. So, what are genes and plasmids and how are they related to bacteria and genetic transformation. (Hanahan, Jessee, & Bloom, Plasmid transformation of Escherichia coli and other bacteria, 1991)Well genes are part of DNA which basically give instructions to make molecules which are called proteins. In this bacteria transformation procedure bacteria are transformed with a gene that has codes for GFP (Green Fluorescent Protein). The bacteria with this gene will cause them to glow a brilliant green color under UV light. So, plasmids carry a gene that contains one or more traits that may be beneficial to survival. In this scenario, the pGLO has a resistance to antibiotics such as ampicillins, which is the one used in this method along with the bacteria E coli because it can easily be grown gene can be turned on in transformed cells by adding arabinose to the cells. SO therefore, the transformed cells will grow on plates with LB/amp and appear white on plates not contain