pGlo transformation

Powerful Essays
Catalog Number
166-0003EDU

Week 7: pGLO Transformation
Introduction to Transformation
In this lab you will perform a procedure known as genetic transformation. Remember that a gene is a piece of DNA which provides the instructions for making (codes for) a protein. This protein gives an organism a particular trait. Genetic transformation literally means change caused by genes, and involves the insertion of a gene into an organism in order to change the organism’s trait. Genetic transformation is used in many areas of biotechnology. In agriculture, genes coding for traits such as frost, pest, or spoilage resistance can be genetically transformed into plants. In bioremediation, bacteria can be genetically transformed with genes enabling them to digest oil spills. In medicine, diseases caused by defective genes are beginning to be treated by gene therapy; that is, by genetically transforming a sick person’s cells with healthy copies of the defective gene that causes the disease.
You will use a procedure to transform bacteria with a gene that codes for Green
Fluorescent Protein (GFP). The real-life source of this gene is the bioluminescent jellyfish Aequorea victoria. Green Fluorescent Protein causes the jellyfish to fluoresce and glow in the dark. Following the transformation procedure, the bacteria express their newly acquired jellyfish gene and produce the fluorescent protein, which causes them to glow a brilliant green color under ultraviolet light.
In this activity, you will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to one large chromosome, bacteria naturally contain one or more small circular pieces of DNA called plasmids. Plasmid DNA usually contains genes for one or more traits that may be beneficial to bacterial survival. In nature, bacteria can transfer plasmids back and forth allowing them to share these beneficial genes. This natural mechanism allows bacteria to

You May Also Find These Documents Helpful

  • Better Essays

    Pglo Transformation

    • 3036 Words
    • 13 Pages

    Introduction In this week’s laboratory period students had the opportunity to perform a common procedure preformed by many if not all microbiologists known as genetic transformation. Genetic transformation is the ability to move DNA into an organism and thereby altering its genotypic and genetic makeup (2). Genetic transformation has shown to have a wide variety of uses in many scientific studies. In agriculture, gene coding for traits such as frost, pest, or spoilage resistance have been genetically…

    • 3036 Words
    • 13 Pages
    Better Essays
  • Better Essays

    Pglo Transformation

    • 1825 Words
    • 8 Pages

    Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab, the Green Fluorescent Protein, which is typically found in the bioluminescent jellyfish Aequorea…

    • 1825 Words
    • 8 Pages
    Better Essays
  • Good Essays

    Pglo Transformation

    • 995 Words
    • 4 Pages

    Miguel Felix pGLO Transformation Mr. Betz AP Biology 14 December 2012 Abstract The purpose of this experiment is to determine the effects of the addition of a plasmid to a bacterial cell. The bacteria E. Coli was separated into two groups: one where the pGLO plasmid was added to the bacteria, which contains the genes of fluorescence and resistance to antibiotics, and the other lacking the plasmid. The two groups then placed in agar plates simulating different environments: the bacteria lacking…

    • 995 Words
    • 4 Pages
    Good Essays
  • Good Essays

    In this lab, we performed a genetic transformation through the process of gene transfer. Gene transfer involves the insertion of a gene into an organism. The gene to be inserted is usually contained in a plasmid, which is relatively small, circular non-chromosomal DNA molecule typically found in bacteria. Once the plasmid containing the gene is inserted into the organism, it is absorbed into the organism’s own genetic code. After this occurs, the newly introduced gene begins coding for proteins,…

    • 702 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Transformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another, through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith, a British microbiologist. Griffith observed that the mutant form, non-virulent form, of the bacteria Streptococcus Pnumoniae could be transformed into the normal, virulent form, when injected into mice along with heat killed normal forms. He concluded that somehow…

    • 1470 Words
    • 6 Pages
    Better Essays
  • Powerful Essays

    Genetic transformation happens when an organism is altered by the introduction of new genetic information which is merged into the organism’s genome. Bacterial transformation is a type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab, the engineered pGLO plasmid is integrated into E. Coli bacteria, and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan, Studies on transformation of Escherichia…

    • 1199 Words
    • 5 Pages
    Powerful Essays
  • Better Essays

    Transformation Of Escherichia Coli With pGLO Plasmid April 24, 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait, in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid, which carries a gene for ampicillin…

    • 1560 Words
    • 7 Pages
    Better Essays
  • Powerful Essays

    pGLO

    • 1313 Words
    • 6 Pages

    Genetic Transformation of Bacteria Abstract The transformation of bacteria was successfully carried out using a plasmid carrying a gene that codes for green fluorescent protein, which gives a signature green glow reminiscent of a jellyfish. This gene, however, is only active when the sugar arabinose is present. A gene coding for antibiotic resistance was also found within the plasmid and served as a means to verify that transformation had indeed taken place. The hypothesis was that the bacteria…

    • 1313 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    Genetic Transformation with pGLO Plasmid Introduction: Genetic transformation is where one organism takes on a characteristic from another organism (Bacterial Transformation 2013). For this experiment we used the bacteria E. Coli to take in foreign jellyfish DNA which will allow it to change genetic material. This experiment determines the effects that the plasmid pGLO has in transferring the Green Florescent Protein found in a jellyfish into the bacteria. It determines whether or not pGLO acts…

    • 1189 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    Introduction Bacterial transformation is the permanent alteration of a bacterial cell genotype as a result of its uptake and incorporation of foreign DNA fragments from external medium (Anthony et al, 2008). In addition to chromosome, bacterial cells often contain extrachromosomal DNA called plasmids which are capable of autonomic replication and antibiotic resistance (Dale & Simon, 2010). Plasmids can transport foreign DNA into host or other bacterial cells hence they are known as vectors.…

    • 1341 Words
    • 6 Pages
    Powerful Essays