Petri Dish Lab Report

Conclusion In part A of this experiment, we transformed the bacteria into an antibiotic resistant form by inserting a plasmid into it. We used heat shock in order to make the bacteria capable to uptake a plasmid in the presence of calcium ions that help disrupt the cell membrane (heat shock is the combination of altering hot and cold). When they are capable of accepting plasmids, the bacteria are incubated with plasmids that carry the resistance to a particular antibiotic, in this case ampicilin. We also ran a control sample along with the experimental one, which is treated the same way except it is not exposed to the plasmid. We then poured the cells onto four Petri dishes that contain Luria broth. Two of the Petri dishes contained the antibiotic,
The second Petri dish, which had plasmids but no antibiotic, also had positive results. The third Petri dish had no plasmid but did contain the antibiotic. No growth occurred in this Petri dish. The last Petri dish had no plasmid and no antibiotic. The results were positive and growth was observed. In Petri dish 1, we found that colonies had formed. Each colony consists of bacteria that have been transformed and are resistant to the antibiotic because they received the plasmid. This Petri dish did glow when under the black light. Petri dish 2 had a lawn of bacteria. It experienced lawn growth because any bacteria would be able to grow on it due of the fact that no antibiotic is present to prevent it. Because it had the plasmid, it did glow when under the black light. Petri dish 3 (the control) had no growth. These bacteria contained the antibiotic and, because they were not incubated with the plasmid resistant to the antibiotic, the antibiotic was able to kill the bacteria. Petri dish 4 had lawn growth because it was incubated with plasmid and no antibiotic to kill the bacteria. Due to this, the bacteria did glow in the black
We placed the lambda DNA into three test tubes. We then incubated one sample with EcoRI, one with HindIII, and the other we left as a control. After we completed the experiment and ran the DNA, we found that the first band of the crime scene DNA traveled 3.5mm and the second band traveled 6.5mm. The actual size of the first crime scene band is 1,100bp and the second band is about 5,500bp. The first band of suspect 1 DNA traveled 3 mm and the second band traveled 6.5mm. The actual size of the first band is 10,000bp and the second band is 5,500bp. Suspect 2 DNA traveled 2.5mm and 7mm. The actual size of the first band is about 1,100bp and the second is about 5,000bp. Because Suspect 1 had bands that traveled the same distance as the crime scene DNA bands, then we were able to conclude that suspect 1 was the criminal. In our expected data, the crime scene DNA traveled 19mm, 21mm, and 32mm. The actual size was 3,800bp, 3,000bp, and 1,000bp. Suspect 1 DNA traveled 21mm, 29mm, and 31mm. The actual size was 3,000bp, 1,300bp, and 1,200bp. Suspect 2 DNA traveled 22mm, 26mm, and 29mm. The actual size was 2,500bp, 1,900bp, and 1,300bp. Suspect 3 DNA traveled 19mm, 21mm, and 32mm. The actual size was 3,800bp, 3,000bp, and 1,000bp. Suspect 4 DNA traveled 20mm, 29mm, and 37mm. The actual size was 3,500bp, 1,300bp, and 8,000bp. Suspect 5 DNA traveled 21mm, 24mm, and 29mm. The