Parkinson's Disease Biochemistry

Nawaf Alhamami
Biochemistry Lab 447 – 002
Dr. Sigrid Jacobshagen
Biochemistry experiment

Research question:
Is Calpains reservation obstructing the Neuronal and Behavioral Shortages in an MPTP Mouse Pattern of Parkinson’s Disease?
Statement of Purpose (Title):
Reservation of Calpains in the prevention of neuronal and behavioral shortages in an MPTP mouse pattern of Parkinson’s disease.
Background information:
In Parkinson’s disease, molecular mechanisms that function to mediate the deterioration of dopamine neurons located at the midbrain not clearly comprehended by many. In the pattern of a mouse of this disease, empirical evidence in support for the role of calcium-dependent protease, in the failure of dopamine neurons is provided
The immunohistochemical examination of postmortem midbrain tissues from the human Parkinson’s disease related cases as well revealed evidence to the improved proteolytic action that is Calpain connected and was not observed in the age-matched monitor subjects. Inferring from the outcomes of this experiment, there is a potentially novel or new relationship that exists between Calpainproteolytic in a MPTP pattern of Parkinson’s disease and the etiology of neuronal loss of the Parkinson’s disease in human beings. A mystery to many is the loss of dopaminergic neuron, because of molecular outcomes, in the substantianigra standards compacta in Parkinson’s disease. Shortage or deficiency in mitochondrial function ascribed to the reduction in complex 1 activity in the SNc, is one popular characteristic of Parkinson’s disease. Practically, landmark structures of Parkinson’s disease alongside the selective dopaminergic neuropathology as well as behavioral deficits are mimicked by the administration of chemical inhibitors of complex 1 of the mitochondrial respiratory chain. Reduction of the proper functioning of mitochondria is linked to stress and research findings reaffirm the accumulation of proof that nigral dopamine neurons are very delicate to it.
Hypothesis
There is a potential
During the first hour observation period, total horizontal ambulatory distance traveled was reported in centimeters. To determine whether the loco motor deficit in this novel environment was reversible with dopamine replacement, additional groups of unlesioned and MPTP-lesioned mice were also administered. In additional groups of animals the administration of amphetamine in activity home cages, outfitted with infrared detector arrays, were used to analyze behavioral performance. To exclude the influence of a novel environment on behavioral performance, home cages were used for amphetamine induced activity. Analyses were conducted by persons ignorant of the investigational