Osmosis Lab Report
Osmosis is a passive movement of water through a selectively permeable membrane moves from an area of higher concentration to an area of lower concentration. In this lab, we can determine the direction of diffusion of small molecules by measuring the diffusion of small molecules through dialysis tubing. This tubing acts as a selectively permeable membrane, and allow to pass larger molecules slowly. Introduction:
Cells have kinetic energy and it causes the molecules of the cell to move around and bump into each other. Diffusion is one result of this molecular movement. Diffusion is the passive movement of molecules from an area of higher concentration to lower concentration. Osmosis is a passive movement of water through a selectively permeable …show more content…
A hypotonic solution would have a high water potential and a low solute potential. An isotonic solution would have equal solute and water potentials. Water …show more content…
The materials needed are dialysis tubing from Fisher Scientific (S413003A) with. We also used four tubes of Spectra/Por® which has a 32mm width and 20.4mm diameter. We also used ~127mm strips. The dialysis tubing has a 12,000 molecular weight cutoff and diameters of 4.8 angstroms. Sucrose has a weight of 342 and solution of 9.3 angstroms. We also need distilled water,
And five 100mL beakers. Start the lab by labeling the beakers 1 through 5, and fill with 4 tap water and one 40% sucrose. Then make up five different solution:2 tap water, 20% sucrose, 40% sucrose, and 60% sucrose. Soak the dialysis tubing and clamp one end of each tube. Pour 10mL of each solution into bags and clamp other end carefully to get any air bubbles out. Rinse off each bag and remove moisture on the surface and record the intial mass of the bags. Fill each beaker with enough water or solution to cover the bag. Weight the bags every 10 min.
Results: Bag Weights (g)
Cells have kinetic energy and it causes the molecules of the cell to move around and bump into each other. Diffusion is one result of this molecular movement. Diffusion is the passive movement of molecules from an area of higher concentration to lower concentration. Osmosis is a passive movement of water through a selectively permeable …show more content…
A hypotonic solution would have a high water potential and a low solute potential. An isotonic solution would have equal solute and water potentials. Water …show more content…
The materials needed are dialysis tubing from Fisher Scientific (S413003A) with. We also used four tubes of Spectra/Por® which has a 32mm width and 20.4mm diameter. We also used ~127mm strips. The dialysis tubing has a 12,000 molecular weight cutoff and diameters of 4.8 angstroms. Sucrose has a weight of 342 and solution of 9.3 angstroms. We also need distilled water,
And five 100mL beakers. Start the lab by labeling the beakers 1 through 5, and fill with 4 tap water and one 40% sucrose. Then make up five different solution:2 tap water, 20% sucrose, 40% sucrose, and 60% sucrose. Soak the dialysis tubing and clamp one end of each tube. Pour 10mL of each solution into bags and clamp other end carefully to get any air bubbles out. Rinse off each bag and remove moisture on the surface and record the intial mass of the bags. Fill each beaker with enough water or solution to cover the bag. Weight the bags every 10 min.
Results: Bag Weights (g)