Osmosis Lab

Topics: Potato, Concentration, Solution Pages: 4 (730 words) Published: March 15, 2014
Investigate the effect of sucrose concentration on osmosis in Solanum tuberosum by recording the change in mass. •Independent Variable: concentration of sucrose (0.0 M, 0.2 M, 0.4 M, 0.6 M, 0.8 M, 1.0 M) (± 0.05) •Dependent Variable: final mass of the cylinders of Solanum tuberosum (± 0.05) •Controlled Variable: temperature, initial length of potato cylinders, volume of the solutions, etc.

Solanum tuberosum (potatoes)
Sucrose solution (0.0 M, 0.2 M, 0.4 M, 0.6 M, 0.8 M, 1.0 M) (± 0.05) •Test tubes and rack
Cork borer
Weighing machine (± 0.05)
Weigh boat
Graduating cylinder
1.Use cork borer to cut 5 potato cylinders each with a length of 3 cm. 2.Determine the initial mass of each cylinder.
3.Place a cylinder in 10 cm3 of distilled water (0.0 M solution). 4.Repeat the same steps for each solution.
5.Cover and leave to stand overnight.
6.Remove cylinders and record the final mass of each.
When the potato cylinders were taken out of distilled water (0.00 M sucrose solution) they expanded and got stiffer. But when they were removed from 0.2 and 0.4 M sucrose solution, they stayed just about the same. Whereas, when they were extracted from the 0.6, 0.8 and 1.0 M sucrose solutions, they were shriveled and soft. They shrunk in size as well.

Raw Data:
Recorded (calculated average) data showing the change in masses of cylinders of Solanum tuberosum due to the effect of different concentrations of sucrose solutions. Concentration of sucrose (± 0.05 M)Mass of Solanum tuberosum (mean of 5 trials for each) (± 0.05 g)

0.00 (distilled water)2.543.18


Processed Data:
Concentration of sucrose (± 0.05 M)Standard deviation σ (correct to 4 d.p) 0.00 (distilled water)0.0837

Cited: Hoong, Tho Lai. "Diffusion and Osmosis." Science 2. Singapore: EPB Pan Pacific, 2007. 76-78. Print.
Kwan, Lam Peng., and Geoff Hayward. "Diffusion, Osmosis and Surface Area : Volume Ratio." Biology: A Course for 'O ' Level. Singapore: Federal Publications, 2004. 23-33. Print.
Ward, William. "Cells; 2.4 Membranes." Pearson Baccalaureate Higher Level Biology. Malaysia: Pearson Education Limited, 2007. 34. Print.
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