Optimum Temperature Of Sucrase

For this experiment, there will be an optimal point for each factor. I believe that the optimal temperature for the enzyme sucrase will be 37°C because that is the point where the temperature increases the rate of reaction to its greatest point without denaturing it. For the pH test, I think that the optimal pH for sucrase will be the pH of 7 because it is neutral so it won’t affect the charge in a negative or positive way. For the denaturation test, I think that the optimal treatment for the enzyme is when both the enzyme and substrate are untreated. For the substrate concentration test, I think that the optimal concentration is 100% because the more the substrate, the faster the enzyme can bind to it. Based on the data above, all four hypotheses were proven correct. Based on the graph and data table, the optimal temperature for sucrase was 37°C because it had a reactivity score of 4, meaning it was very reactive. For the pH test, based on the graph and data table, the optimal pH was in fact ranged from 3-7. This means that any pH from 3-7, gives the highest rate of reaction to sucrase. The denatured test was proven correct because as the hypothesis said, if the enzyme and substrate are both untreated, the rate of reaction will be the highest and based on the graph and data table, this was the case.