Ocean Catfish

We have transferred two channel catfish Ictalurus punctatus growth hormone cDNA constructs (opAFP-ccGH and rtMT-ccGH) driven by the ocean pout Zoarces americanus antifreeze protein promoter (opAFP) or rainbow trout Oncorhynchus mykiss metallothionein promoter (rtMT) via electroporation to channel catfish to produce transgenic catfish by introducing additional copies of growth hormone cDNA and compered the inheritance of the GH transgenesis, and growth rate.
Most of the DNA constructs are lost during the first 10 days after delivery into host embryos. Only about 0.0001% of the construct take permanent residence in the fish genome. Integration rates between 2% to 30% have been reported (Chen and Powers 1990; Hackett 1993; Karsi 2001; Chiou et
2004, 2006; Hobbs and Fletcher 2008; Raven et al. 2008; Higgs et al. 2009; Leggatt et al. 2012; Tibbetts et al. 2013; Devlin et al. 2015; Abass and Dunham 2015, 2016a,b). Dunham et al. (2002) reported that the transgenic common carp Cyprinus carpio by rainbow trout cDNA in the four families of the F1 generation and eight families in the F2 generation grew 3% to 37% and 0% to 49% faster than their non-transgenic siblings, respectively. At one year old, the transgenic Atlantic salmon by Chinook salmon GH cDNA driven by the ocean pout antifreeze protein gene grew 2 to 6 fold larger than non-transgenic control and the largest transgenic fish grew 13 times that of the average non-transgenic (Du et al. 1992) and Oreochromis niloticus transgenic by Chinook salmon GH cDNA driven by the ocean pout antifreeze protein grew 2.5 to 4 fold faster than non-transgenic siblings (Rahman et al. 1998, 2001; Rahman and Maclean 1999; Caelers et al.
GloFish, fluorescent zebrafish is the first transgenic fish to be commercially applied for ornamental purposes. FDA (2015) have been approved triploid Atlantic salmon GH transgenic for the first time for human consumption. This approval will open the gate to other transgenic GH meat produce for human