Nt1310 Unit 3 Pathogens

1. Make sure you label both plates on the bottom, with your name, date and name of pathogen you have chosen (listed above under materials) Then label numbers 1-5 on one plate and 6-10 on the other as there are 10 disks that need to be placed. Spread out in a circle formation like pictured below (This is the final product; look at this for reference on how to label)

Now you are ready to start culturing. Start with one of your plates you can do both at the same time but to avoid making any errors I recommend just doing one at a time. Using aseptic technique take the tube containing the pathogen you have chosen and sterilize it. Next, take your sterile pipette (dropper) and pick up a small amount of your sampled pathogen. Using aseptic technique again flame the tube and close it, set aside till you are ready to use for the next plate. Open the lid of the first plate (I used the one labeled 1-5 first)
Then drop 3 drops of the sampled pathogen from the pipette into the plate. Close the lid and discard the pipette into the clear bag with the red label on it. Then take one of the sterile spreaders (looks like a hockey stick) open the same plate aseptically and spread the three drops you put in all around the plate. Be careful not the press and damage the broth. After you have spread the pathogen around close the lid and throw away the hockey stick in the biohazard bag. Repeat for the next plate you labeled 6-10. The final step of the first day you will place the disks containing various antibiotics (known concentrations). Aseptic technique is used when picking up each disk and putting it into the plate. Meaning you need to flame the forceps before and after you pick up and put in the disk in while also opening the plates away from you. Each plate that contains the concentrated disks is labeled with a number match that number with the number you have labeled on the bottom of your