Nasturtium Officinale Case Study
Further evidence for thein-vivo anti-inflammatory activity of Nasturtium officinale
Abstract:
Ethnopharmacological relevance:Aerial parts of Nasturtium officinaleR. Br. are used traditionally in Iranas a folk medicinein different illnesses which includebronchitis, asthma, diabetes, hypertension, and renal colic.
Aim of the study:In the present study,the anti-inflammatory activity of hydro-alcoholicextract from aerial parts of N.officinalewas investigated in acute and sub¬¬-acute models of inflammation.
Materials and methods:Different models like carageenan induced hind paw edema in rats, formalin induced paw edema in rats and 12-O-tetradecanoylphorbol-13-acetate (TPA)evoked ear edema in mice were used for studying the anti-inflammatory activity …show more content…
The levels ofTNF-α and IL-1β in the whole skin of inflamed paws were determined as described previously (Nacife et al., 2004) by enzyme-linked immunosorbent assay (ELISA). The tissue samples were weighed; snap frozen on liquid nitrogen and stored at −70 °C to be processed for IL-1β and TNF-αdeterminations. Skin tissue was homogenized in phosphate buffered saline (PBS; pH=7.4) containing 0.4 M NaCl, 0.05% Tween-20, 0.5% bovine serum albumin, 0.1 mMphenylmethylsulfonyl fluoride, 0.1 mMbenzethonium chloride, aprotinin A 20 KI, and 10 mM EDTA. The homogenateswere centrifuged at 12,000×g for 30 min at 4 °C, and then ELISA was used to measure the levels of IL-1β and TNF-α in the …show more content…
Next, the removed stomach of each animal was cut through the greater curvature. Finally, the mucosal surface was washed with normal saline and observed with a convex lens for possible injuries or bleeding (Akkol et al., 2008).
2.11. Acute toxicity
In order to determine the plant LD50, three groups of rats (n= 6) received the hydro-alcoholic extract (1, 3 and 5 g/kg; p.o.). The animals were observed during 48 h (Lorke, 1983).
2.12. Histological examination
For histopathological examination,three samples of ears or paws from the control and extract treated animals were taken and fixed in 10% formaldehyde solution for 1 week. Then, the fixed biopsies were embedded in paraffin and cut into 3–4 mm slices. The slices were mounted on glass slides and stained with hematoxylin and eosin for light microscopy analysis. The assessment was conducted by a pathologist in a blindedway.
2.13. Statistical analysis
The data were expressed as the means±SEM. The differences between the control and treatment groups were tested by ANOVA followed by the Tukey post-hoc test, using SPSS 13.0 software. The probability of p<0.05 was considered to show considerable differences for all comparisons
Abstract:
Ethnopharmacological relevance:Aerial parts of Nasturtium officinaleR. Br. are used traditionally in Iranas a folk medicinein different illnesses which includebronchitis, asthma, diabetes, hypertension, and renal colic.
Aim of the study:In the present study,the anti-inflammatory activity of hydro-alcoholicextract from aerial parts of N.officinalewas investigated in acute and sub¬¬-acute models of inflammation.
Materials and methods:Different models like carageenan induced hind paw edema in rats, formalin induced paw edema in rats and 12-O-tetradecanoylphorbol-13-acetate (TPA)evoked ear edema in mice were used for studying the anti-inflammatory activity …show more content…
The levels ofTNF-α and IL-1β in the whole skin of inflamed paws were determined as described previously (Nacife et al., 2004) by enzyme-linked immunosorbent assay (ELISA). The tissue samples were weighed; snap frozen on liquid nitrogen and stored at −70 °C to be processed for IL-1β and TNF-αdeterminations. Skin tissue was homogenized in phosphate buffered saline (PBS; pH=7.4) containing 0.4 M NaCl, 0.05% Tween-20, 0.5% bovine serum albumin, 0.1 mMphenylmethylsulfonyl fluoride, 0.1 mMbenzethonium chloride, aprotinin A 20 KI, and 10 mM EDTA. The homogenateswere centrifuged at 12,000×g for 30 min at 4 °C, and then ELISA was used to measure the levels of IL-1β and TNF-α in the …show more content…
Next, the removed stomach of each animal was cut through the greater curvature. Finally, the mucosal surface was washed with normal saline and observed with a convex lens for possible injuries or bleeding (Akkol et al., 2008).
2.11. Acute toxicity
In order to determine the plant LD50, three groups of rats (n= 6) received the hydro-alcoholic extract (1, 3 and 5 g/kg; p.o.). The animals were observed during 48 h (Lorke, 1983).
2.12. Histological examination
For histopathological examination,three samples of ears or paws from the control and extract treated animals were taken and fixed in 10% formaldehyde solution for 1 week. Then, the fixed biopsies were embedded in paraffin and cut into 3–4 mm slices. The slices were mounted on glass slides and stained with hematoxylin and eosin for light microscopy analysis. The assessment was conducted by a pathologist in a blindedway.
2.13. Statistical analysis
The data were expressed as the means±SEM. The differences between the control and treatment groups were tested by ANOVA followed by the Tukey post-hoc test, using SPSS 13.0 software. The probability of p<0.05 was considered to show considerable differences for all comparisons