Molecular Phylogeny of the Endangered Vietnamese Paphiopedilum Species Based on the Internal Transcribed Spacer of the Nuclear Ribosomal DNA
K. H. Trung1, T. D. Khanh2 , L. H. Ham2, T. D. Duong2 and N. T. Khoa2
Genetic Engineering Lab, Agricultural Genetics Institute, Hanoi, Vietnam
Molecular Biology Division, Agricultural Genetics Institute, Hanoi, Vietnam Corresponding author: firstname.lastname@example.org
Copyright © 2013 K. H. Trung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract We have sequenced the internal transcribed spacer (ITS) region (including ITS1, 5.8S, and ITS2) of 16 species and two varieties of the slipper orchid genus Paphiopedilum in Vietnam. The identification of relationship between those species was also assessed using molecular tool. Most of slipper orchid species in Vietnam are now endangered or threatened to extinction due high demand of ornamental trade of these beautiful species. However, it is difficult to distinguish them without their flowers. Therefore, it is needed to develop molecular markers to accurately identify these species. Our present study is in the view to set the first steps in constructing a molecular database of slipper orchids for future assessment of inter- and intra-specific molecular diversity of Paphiopedilum species from Vietnam, to contribute to the conservation of these valuable orchids. The results showed that highly variable areas (that contain many indels) in ITS region are useful for phylogenetic analysis as the dataset containing these long-indels areas and gave better Jack-knife supports in resolving the relationships of Paphiopedilum species than the modified