Preview

Molecular detection of fungal pathogens.

Powerful Essays
Open Document
Open Document
1440 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Molecular detection of fungal pathogens.
MOLECULAR DETECTION OF FUNGAL DISEASES

INTRODUCTION

With the rise of many new diseases caused due to viruses, bacteria and fungi; it is essential for the rapid detection of such diseases. The severity of such diseases can be reduced by its rapid detection carried out by different methods. The conventional methods may include the idea of just identifying the disease symptoms, identification of these pathogens in the laboratory by different morphological and biochemical tests, etc [1]. The conventional methods may include certain disadvantages like incorrect identification which would eventually lead to an incorrect diagnosis and a wrong treatment. Certain other factors also need to be kept in mind like:-[1,3]
Requirement of highly skilled laboratory personnel,
High risk of contamination,
Time consuming,
Antigenic cross reactivity between species and genera,
Possibly hazardous cultures, and,
Non – quantitative.

Keeping the above points with respect to the conventional methods, rapid molecular diagnostic tests have been developed. Molecular diagnostic tests include PCR, immunoassays, and DNA/RNA probe technology [1].

FUNGAL DISEASES

Unlike viral pathogens which are breaking headlines in the news, it has often been said that fungal diseases are a silent epidemic [2]. Fungal diseases normally affect immune – compromised hosts, patients requiring complex surgical procedures like in trauma cases, or in patients hospitalized with severe diseases like acute myelogenous leukemia. Aspergillus fumigatus and Candida albicans are the major cause invasive filamentous infections and other yeast related infections [2]. The centre for disease control and prevention has categorized fungal infections into three main categories –
1) Opportunistic infections – These infections affect people suffering from severe diseases which have weakened the immune system. Examples include cancer, HIV/AIDS, transplant recipients etc. Such people usually suffer from

You May Also Find These Documents Helpful

  • Good Essays

    Microbiology Unknown

    • 2745 Words
    • 11 Pages

    The objective of this project was to identify an unknown bacterium through several differential tests. Identifying an organism can be very beneficial in the field of medicine. Knowing what an organism is can indicate its pathology and treatment. These tests are designed to find out about an organism’s metabolism and other various characteristics. For this project, ten…

    • 2745 Words
    • 11 Pages
    Good Essays
  • Good Essays

    The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed, provided us with some key information about the unknown microbes in question .…

    • 698 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Outbreak Movie Analysis

    • 1021 Words
    • 5 Pages

    Sixth and final link is the susceptible host. There are several factors that can influence the severity of the infection. The new host immune system, age (very young or old), medical conditions, prior treatments or invasive devices, and the overall general health and nutrition of the new…

    • 1021 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Infection due to a person's health which can deteriorate very quickly due to a chest or urinary tract infection (UTI), dental problems, foot problems that are commonly associated with diabetes, elderly people with…

    • 731 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Fungal Meningitis

    • 1464 Words
    • 6 Pages

    Andes D and Casadevali A. 2013. Insights into fungal pathogenesis from the iatrogenic epidemic of Exserohilum rostratum fungal meningitis. Journal of fungal genetics and Biology. Vol 61: 143-145…

    • 1464 Words
    • 6 Pages
    Better Essays
  • Satisfactory Essays

    PLTW 1.2.2

    • 431 Words
    • 2 Pages

    Extract DNA of virus from infected cell and run PCR. It will be the same process done with the bacterial DNA. You can also check symptoms. You can then use the sequenced DNA and compare it to the DNA of various viruses.…

    • 431 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Bacteria - Bacteria are organisms made up of just 1 cell. They are capable of multiplying by themselves, as they have the power to divide. Bacteria exist everywhere, inside and on our bodies. Most of them are completely harmless and some of them are very useful. But some bacteria can cause diseases, either because they end up in the wrong place in the body or simply because they are 'designed' to invade us.…

    • 1172 Words
    • 5 Pages
    Good Essays
  • Better Essays

    In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is a fermenter they will use the sugars to make ATP. If the bacteria is a fermenter of lactose/sucrose the EMB plate we used will “clearly differentiate between the colonies of lactose fermenting and non-fermenting microbes. In the same medium sucrose was also included to differentiate between coliforms that were able to ferment sucrose more rapidly than those that were unable to ferment sucrose” (Cheeptham & Lal, 2007).…

    • 1370 Words
    • 6 Pages
    Better Essays
  • Better Essays

    the foreign DNA successfully inserted can be easily identified from the nonrecombinants based on the colour difference of colonies on growth media.…

    • 1878 Words
    • 27 Pages
    Better Essays
  • Satisfactory Essays

    Colonisation - is when a bacterial strain invades a region of your body and starts to rapidly divide - it sets up a new colony…

    • 302 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Infection Control

    • 1774 Words
    • 8 Pages

    - Sharing infected needles. - Having unprotected sexual activities. - Unhygienic practices when dealing with individuals (not washing hands after assisting with personal and sanitary care). - The use of dirty medical instruments. - Underlying medical conditions and heredity.…

    • 1774 Words
    • 8 Pages
    Good Essays
  • Better Essays

    Unknown Lab Report

    • 2145 Words
    • 9 Pages

    The tests performed on the unknown bacteria cultures were all used to determine the identity of the bacteria. Each of the tests performed provided some key information about the bacteria in question and how it functions. Not all of the tests were performed on every culture, however, as some of the tests were used only for gram (+) or gram (–) bacteria, while others were even more specific and used only for cocci bacteria. The tests performed and what constitutes a positive and negative test are as follows.…

    • 2145 Words
    • 9 Pages
    Better Essays
  • Satisfactory Essays

    Modern tests swab the targets hands or clothing and analyze the results using scanning electron microscope (SEM) analysis test.…

    • 268 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    4. Risk for infection inadequate primary defenses, (broken skin, traumatized tissue, changes in pH secretions), malnutrition, tissue destruction…

    • 1145 Words
    • 6 Pages
    Good Essays
  • Good Essays

    Hiv Infections Case Study

    • 546 Words
    • 3 Pages

    List other major life activities that may be limited when a person has an infectious disease.…

    • 546 Words
    • 3 Pages
    Good Essays