Did you observe any pressure changes during this experiment? If so, in which beaker(s), and with which membranes? In the L beaker with the 20 MWCO Membrane, the pressure went up. Why? Because no diffusion took place.
Did the Na?/Cl? diffuse from the left beaker to the right beaker? If so, with which membrane(s)? Yes, 50, 100, and 200 MWCO Why? Because diffusion took place.
Explain the relationship between solute concentration and osmotic pressure. They are directly proportional, if the solute concentration increases the osmotic pressure will increase. Does diffusion allow osmotic pressure to be generated?
If? solutes ?are ?able ?to ?diffuse,?then ?equilibrium ?will? be ?established and osmotic ?pressure ?will ?not ?be? generated. Would pressure be generated if solute concentrations were equal on both sides of the membrane? Osmotic ?pressure ?would? be ?zero ?if? albumin ?concentration ?was ?the same? on ?both? sides ?of ?the? membrane. Why or why not? If? you ?increased? (or ?doubled) ?the ?concentration ?of ?albumin,?osmotic? pressure ?will increase ?(or ?double). Would pressure be generated if you had 9 mM glucose on one side of a 200 MWCO membrane and 9 mM NaCl on the other side? If so, which solution was generating the pressure? No, because both glucose and NaCl are diffusible with a 200 MWCO membrane. Would pressure be generated if you had 9 mM albumin on one side of a 200 MWCO membrane and 9 mM NaCl on the other side? If so, which solution was generating the pressure? Yes, the albumin would generate the pressure, because it does not diffuse.
ACTIVITY 5: Filtration
What were the results of your initial membrane analysis? Solute Residue for all solutes (Na/Cl, Urea, Glucose, Powdered charcoal) were present. Does the membrane MWCO affect filtration rate? Yes, the larger the MWCO the faster the filtration Does the amount of pressure applied affect the filtration rate? Yes, the greater the pressure, the faster the filtration Did all solutes pass...
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