Preview

Mitochondria Lab Report

Powerful Essays
Open Document
Open Document
3238 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Mitochondria Lab Report
Experiment IV: Study of Krebs cycle using Mitochondria from Mung Bean Seedlings

INTRODUCTION
The mitochondria is a very important organelle in the plant cell because it carries out very important cellular reactions in the cell like the Krebs cycle and oxidative phosphorylation which is how the plants produce ATP from the pyruvate produced through glycolysis (Meyer and Millar, 2008). Glycolysis produces a net of 2 ATP for the plant which is not enough for the cell to function while the Krebs cycle and electron transport chain produces a net of 36 ATP which makes the mitochondria the power house of the plant cell (Meyer and Millar, 2008). Mitochondrion is also an important organelle because it contains its own genome and can be transcribed
…show more content…
In addition isolated mitochondria are studied to identify their proteomes and how development affects the proteome (Labbe et al., 2008). Study of isolated mitochondria has great application in the pharmaceutical industry through the discovery that some mitochondrial proteins stimulate apoptosis in the cells (Wang, 2001). This research has aided in finding cure for cancer and Parkinson’s disease among many other diseases (Labbe et al., 2008). Also, many drugs produced contain drug classes that can inhibit the function of mitochondria by preventing or altering a stage in ATP production in the Krebs cycle or electron transport chain or acting as an inhibitor of an enzyme that carries out a certain reaction in citric acid cycle or ETC (Dykens and Will, 2007). These drugs are therefore pretested …show more content…
W., M. G. Montgomery, G. W. Andrew & J. E. Walker. 2006. How azide inhibits ATP hydrolysis by the F-ATPases. PNAS. 103: 8646-8649
Brummond, D.O., and R.H. Burris. 1954. Reaction of trycarboxylic acid cycle in green leaves. Journ. Biol. Chem. 209: 755-765.
Dykens, J. A., and Y. Will. 2007. The significance of mitochondrial toxicity testing in drug development. Drug Discovery Today. 12(18): 777-785.
Galbraith, D.W., D.P. Bourque & H.J. Bohnert. 1995. Isolation and fractionation of plant mitochondria and chloroplast. Methods in Cell Biology. 2: 161-173.
Gross, V. S., H. K. Greenberg, S. V. Baranov, G. M. Carlson, I. G. Stravovskraya, A. V. Lazarev & B. S. Kristal. 2011. Anal Biochem. 418(2): 213-223.
Labbe, G., D. Pessayre & B. Fromenty. 2008. Drug-induced liver injury through mitochondrial dysfunction: mechanisms and detection during preclinical safety studies. Fundamentals in Clinical Pharmacology. 22(4): 335-353.
Meyer E. H., and A. H. Miller. 2008. Isolation of mitochondrial from plant cell culture. Methods in molecular Biology. 425:

You May Also Find These Documents Helpful

  • Good Essays

    The mitochondria is the site of ATP production for the cell. ATP is the energy currency of the cell. Mitochondria are nicknamed, the "powerhouse".…

    • 412 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    BIO 104 Chapter 3

    • 7229 Words
    • 29 Pages

    Mitochondria carry out critical steps in the extraction of energy from food, and the conversion of that “trapped” energy to a useful form. They are the cell’s “power plants.” Chloroplasts are organelles found in algae and in the green parts of plants. Chloroplasts have two membranes surrounding them, as well as an internal system of stacked membrane discs.…

    • 7229 Words
    • 29 Pages
    Powerful Essays
  • Good Essays

    Mitochondria have a "bag within a bag" structure. This is necessary to: create two distinct regions with a concentration gradient between them, a form of potential energy.…

    • 500 Words
    • 2 Pages
    Good Essays
  • Good Essays

    The mitochondria is the powerhouse of the cell it provides energy to the cell through respiration.The food that we eat is broken into simpler molecules like carbohydrates, fats and etc in our bodies. These are sent to the mitochondrion where they are further precessed to produce charged molecules that combine with oxygen and produce Adenosine TriPhosphate (ATP) molecules. This entire process is known as oxidative phosphorylation.Mitochondria also helps in the building of certain parts of the blood, and hormones like testosterone and estrogen.…

    • 656 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Mitochondria is the second largest organelle with unique genetic structure and it is responsible for metabolism of…

    • 835 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    Meisenberg G, Simmons WH: Principals of Medical Biochemistry; 2nd Edition, 2006, Mosby Elsevier: PA, USA.…

    • 1779 Words
    • 8 Pages
    Powerful Essays
  • Satisfactory Essays

    End Products: 4CO2 + 2 ATP + 6 NADH + H+ +2 FADH2 *Oxaloacetate is regenerated…

    • 668 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Unit 5 P1

    • 497 Words
    • 2 Pages

    Mitochondria- Mitochondria are the energy factories of the cells. The energy currency for the work that animals must do is the energy-rich molecule adenosine triphosphate (ATP). The ATP is produced in the mitochondria using energy stored in food. Just as the chloroplasts in plants act as sugar factories for the supply of ordered molecules to the plant, the mitochondria in animals and plants act to produce the ordered ATP molecules as the energy supply for the processes of life.…

    • 497 Words
    • 2 Pages
    Good Essays
  • Better Essays

    Case Study 2 WwWL

    • 2400 Words
    • 7 Pages

    The main function of the mitochondria is to convert fuel into a form of energy the cell can use. Specifically, the mitochondria is where pyruvate --derived from glucose-- is converted into ATP (Adenosine triphosphate) through cellular respiration. Cellular respiration involves four stages: glycolysis, the grooming phase, the citric acid cycle, and oxidative phosphorylation. The final two stages listed occur in the mitochondria.…

    • 2400 Words
    • 7 Pages
    Better Essays
  • Satisfactory Essays

    Assay for Cytochrome oxidase activity. (+ purple) Shows the effects from the addition of NADI, a very…

    • 592 Words
    • 3 Pages
    Satisfactory Essays
  • Powerful Essays

    Respiration is a very important process for every living organism. While it is typically thought of as breathing in oxygen, and exhaling carbon dioxide, like all things, it must take place at the cellular level. The electron transport chain is responsible for cellular respiration. The process uses four complexes; the fourth is cytochrome c oxidase. Cytochrome C oxidase is responsible for the reduction of oxygen to water. There are thirteen subunits that make up the complete complex. Each of these is coded by its own gene in the DNA. Most of them are in the nuclear DNA; however three are encoded by mitochondrial DNA, including COX1 (Karp, 2010) (Li, Youfen et al.).…

    • 2049 Words
    • 9 Pages
    Powerful Essays
  • Powerful Essays

    Figure 4 - Bailey, R. (2012) About.Com Website. Available at: http://biology.about.com/od/cellanatomy/ss/mitochondria.htm (Accessed 5th March 2013).…

    • 1729 Words
    • 7 Pages
    Powerful Essays
  • Good Essays

    class note

    • 1830 Words
    • 15 Pages

    The Chemiosmotic Theory Lec 10 B. ATP Synthase C. The P/O ratio D. Uncoupling oxidative phosphorylation 4. Control of ATP Production A. Control of oxidative phosphorylation B. Coordinated control of oxidative metabolism Lec 11 5. Physiological implications of aerobic metabolism A. Cytochrome P450 B. Reactive Oxygen Species C. Antioxidant Mechanisms Mitochondria and chloroplasts are organelles of energy conversion that carry their own DNA Mitochondria – release energy from nutrients and convert it to ATP Chloroplasts – capture solar energy and store it in carbohydrates Organelles in a cell Mitochondria Mitochondria are membrane-enclosed organelles distributed through the cytosol of most eukaryotic cells.…

    • 1830 Words
    • 15 Pages
    Good Essays
  • Powerful Essays

    Unit two Biology

    • 7492 Words
    • 30 Pages

    The space between the two mitochondrial membranes usually has a lower pH than the matrix of the mitochondrion as a result of the H+ that are transported into the intermembrane space by the activity of the electron transport chain. They are contained there until they are allowed out during ATP synthesis. These cristae give the inner membrane a large total surface area, so it can hold many molecules of the electron transport chain and ATPase. The more membrane there is, the more ATP and reduced NAD can be produced. Cristae in mitochondria from different types of cells show considerable variation in appearance, but, Figure 2.12 TEM of a mitochondrion inside a pancreatic cell, where much ATP is required for the synthesis of enzymes.…

    • 7492 Words
    • 30 Pages
    Powerful Essays
  • Better Essays

    Raven, P., Evert, R., and Eichhorn, S. 1999. Biology of Plants. Freeman & Co.: New York. 6th ed.…

    • 964 Words
    • 4 Pages
    Better Essays