Microscope lab report
Microscope is a tool used to enlarge images of small objects that are hard to study with bare eyes. The compound light microscope, which is going to be used in this lab activity, is an instrument with two lenses and various knobs to focus the image. In this lab, we will learn about the proper use and handling of the microscope. Objectives:
•Demonstrate the appropriate procedures used while using the compound light microscope correctly. •Make and use a wet mount.
•Calculate the total magnification of the microscope.
•Explain how to handle the microscope properly.
•Describe changes in the field of view and the amount of light when going from low to high-power objectives using the compound light microscope. •Explain why objects must be centered in the field of view before changing from low to high-power objective. •Explain how to control the light intensity when changing the power of objectives. •Explain the proper process for focusing under low and high-power using the compound light microscope. Procedures
•Beaker of water
•The letter “e” cut from newsprint
•Plant or algae specimens
1. Microscope should be treated with care; put one hand on the arm and the other under the base of the microscope when carrying it. 2. Carry one microscope carefully and properly from the microscope storage area to the working area. 3. Pick up a pair of scissors, newsprint, a slide, and a coverslip. 4. Remove the dust cover of the microscope and set it properly. 5. Examine the microscope and give the function of each of the parts.
-Contains a magnifying lens
-Contains a magnifying lens
4 - opening of the - Supports the body tube Stage
5 - fine adjustment - Allows light to pass up to the
6 - coarse adjustment -adjust magnification Knob
7 - base -holds scope and components
8 - Illuminator -reflects light
9 - Diaphragm -controls the amount of light
10 - diaphragm lever -opens and closes diaphragm
11 - stage clips -holds the slide
12 - low-power -10x magnification Objective
13 - high-power -100x magnification Objective
14 - nosepiece -holds objectives, can be rotated
15 - body tube - Keeps distance between the eyepiece And the objectives.
Preparing a wet mount of the letter “e”.
1. Cut out letter “e” from the newspaper.
2. Place it on the glass slide.
3. Cover it with a clean cover slip so it looks like:
4. Place a drop of water on the edge of the cover slip using the eyedropper. 5. Turn on the microscope and place the slide on the stage.
There is a rule for determining total magnification of a compound microscope. To find out the total magnification, multiply the number on the eyepiece magnification and the number on the objective magnification. For instance, the total magnification of a low power objective:
In this lab activity, I observed not only...
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