Microbiology Lab Report
Introduction
The science of microbiology is all about microorganisms, what they do and how they work. Microorganisms are ubiquitous, and are a very important part of life everywhere since they have been around for billions of years. The two main themes associated with microbiology are (1) having an understanding of the nature and function of the microbial world, and (2) being able to apply that understanding of the microbial world so that the benefits to the Earth and humans are known(6). This lab specifically does not require the knowledge of what each organism does when introduced to another living organism, like a human. Figuring out the type of bacteria could help with further experiments.
The idea of the unknown bacteria lab is to show …show more content…
In each tube a different kind of unknown bacteria was growing on the agar slant. Having the slanted agar allows for more surface area, therefore, more room for bacteria to grow. For each test there is a general set of rules to follow when transferring bacteria from one culture to another.
Disinfect the table surface.
Sterilize the transfer loop using the burner before and after using it to transfer any bacteria at any point in time, the metal should be red hot.
Always pass the top of the tube through the fire after taking the lid off, and before you put the lid back on.
Never set the lids of the tubes or plates on the table when removed for inoculation of bacteria(5).
The first thing to check off the list was the gram stain and the shape of the bacteria. Next, the lab manual gave further specifics for each type of biochemical tests(5). Except for determining the shape and the gram stain, the rest of the tests are on agar plates, in agar tubes, and in broth tubes. The name of each test performed are listed below in the order the tests were done in the lab. …show more content…
This part is one of the most important things to do correctly. If you don't, you will not have viable bacteria to use for the next set of tests and the whole thing will be ruined. To find whether the bacteria was gram negative or gram positive, there is a specific way to put the bacteria on a slide and stain it so that the color is either pink/red or purple/blue. Finding these two things eliminated the ones on the list that were gram positive and/or were cocci shape, and led to beginning the many biochemical
The science of microbiology is all about microorganisms, what they do and how they work. Microorganisms are ubiquitous, and are a very important part of life everywhere since they have been around for billions of years. The two main themes associated with microbiology are (1) having an understanding of the nature and function of the microbial world, and (2) being able to apply that understanding of the microbial world so that the benefits to the Earth and humans are known(6). This lab specifically does not require the knowledge of what each organism does when introduced to another living organism, like a human. Figuring out the type of bacteria could help with further experiments.
The idea of the unknown bacteria lab is to show …show more content…
In each tube a different kind of unknown bacteria was growing on the agar slant. Having the slanted agar allows for more surface area, therefore, more room for bacteria to grow. For each test there is a general set of rules to follow when transferring bacteria from one culture to another.
Disinfect the table surface.
Sterilize the transfer loop using the burner before and after using it to transfer any bacteria at any point in time, the metal should be red hot.
Always pass the top of the tube through the fire after taking the lid off, and before you put the lid back on.
Never set the lids of the tubes or plates on the table when removed for inoculation of bacteria(5).
The first thing to check off the list was the gram stain and the shape of the bacteria. Next, the lab manual gave further specifics for each type of biochemical tests(5). Except for determining the shape and the gram stain, the rest of the tests are on agar plates, in agar tubes, and in broth tubes. The name of each test performed are listed below in the order the tests were done in the lab. …show more content…
This part is one of the most important things to do correctly. If you don't, you will not have viable bacteria to use for the next set of tests and the whole thing will be ruined. To find whether the bacteria was gram negative or gram positive, there is a specific way to put the bacteria on a slide and stain it so that the color is either pink/red or purple/blue. Finding these two things eliminated the ones on the list that were gram positive and/or were cocci shape, and led to beginning the many biochemical