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Medical Unknown

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Medical Unknown
** Introduction :
A medical microbiology lab performs testing on human samples collected from different body sites. The tests are used to detect and identify any microorganisms capable of causing disease. Knowing of unknown microorganism is important on how this microorganism works and how it is structured, means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory class.

**Materials :
1) Blood agar plate .
2) Mannitol Salt agar (MSA) plate.
3) DNase agar plate .
4) Novobiocin disc .
5) Inoculating loop.
6) flame ( Bunsen burner) .
7) 1N hydrochloric acid (HCl) .
8) Two slides .
9) Plasma tube.
10) 3% Hydrogen Peroxide (H2O2) .
11) One unknown plate .
12) Crystal violet.
13) Gram’s iodine .
14) Safranin.
15) Alcohol .

** Methods :
An unknown labeled with number 8 was given out by the lab instructor. The goal at this point was to determine unknown gram positive vacteria. The procedures performed consisted of sterile technique in addition to being followed as stated in the referenced course laboratory manual by Matar (1) , unless otherwise noted.
Not all of the tests were performed on every culture. However, there are as some of the tests were used only for gram (+) others were even more specific and used only for cocci bacteria .The first procedure have been done was to observe and record the morphology of the unknown sample. However, Gram stain should be done to be sure that unknown sample were gram positive and to identify cells morphology. After that biochemical tests were chosen for unknown identification . first of all was done the catalase test to differentiate between the two types of cocci bacteria ( Staphylococcus and Streptococcus ) . Since unknown 8 was determined to be Staphylococcus coagulase test in addition to the following tests were performed on this



References: : 1) Matar, Suzan. Medical microbiology Laboratory Manual. Jordan: University of Jordan publishing. 2) http://www.studymode.com/subjects/unknown-lab-report-on-gram-positive-bacteria-page1.html . 3) http://en.wikipedia.org/wiki/Gram-positive_bacteria . 4) http://www.ehow.com/about_5453276_staphylococcus-saprophyticus-infection.html

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