Mark Recapture Method

Topics: Ultraviolet, Agar plate, Plasmid Pages: 15 (2276 words) Published: December 2, 2014


methods of population estimation
November 18, 2014

BIOL 1121: General Biology II Lab
Fall 2014

Abstract
Mark and recapture is a method commonly used in ecology to estimate an animal population's size. A portion of the population is captured, marked, and released. This lab provides methods that can be used to estimate a

provided additional information for a better interpretation of lichen diversity values in biomonitoring studies of air pollution.

Introduction
This section is an introduction to the lab objectives and its practical uses. It should normally be 1 to 2 paragraphs in a short report. The primary functions of an introduction are to describe the objectives of this laboratory exercise. The section needs to introduce the lab function and background. Any new concepts and terminology need to be given as well. Last should be the objectives of the lab. Include what should be accomplished or learned through performing this experiment. For example, what is gel filtration chromatography? What does egg white consist of? What were you trying to getting out of the egg white sample? What properties of the technique did make it suitable for your purpose? What did you expect from the results? Why did you fractionate the protein? What did you do with the fractionated protein?

Materials and methods
Part I
Two empty, sterile 1.5 ml microcentrifuge tubes and another one containing 600 µl of transformation solution (CaCl2) were obtained. One of the closed microcentrifuge was labeled +pGLO and the other –pGLO and then placed on a microcentrifuge tube rack. 250 µl of the transformation solution was then transferred to the tubes using a P-1000 micropipette with a sterile tip. The tubes were then placed in ice as the bacterial suspension was being prepared. 2 large (~2 mm in diameter) or 3 small colonies (c) 200 µl of +pGLO cells onto the LB/Amp plate
d) 200 µl of +pGLO cells onto the LB/Amp/ara plate
A new sterile L-shaped spreader...

References: Dept. Biol. Sci. 2012. General Biology Laboratory I Manual. Dept. Biol. Sci., University of Memphis, Memphis, TN.
Biotechnology Explorer Team. 2011. Biotechnology ExplorerTM pGLOTM Bacterial Transformation Kit (Catalog No. 166-0003-EDU) Intruction Manual, Rev G. Bio-Rad Laboratories, Hercules, CA.
Chen, I., and D. Dubnau. 2004. DNA uptake during bacterial transformation. Nat Rev Microbiol. 2:241-249.
Griffith, F. 1928. The significance of pneumococcal types. J Hyg (Lond). 27:113-159.
Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 166:557-580.
Johnson, W.G., V.M. Davis, G.R. Kruger, and S.C. Weller. 2009. Influence of glyphosate-resistant cropping systems on weed species shifts and glyphosate-resistant weed populations. Eur J Agron. 31:162-172.
Nilsson, J., P. Jonasson, E. Samuelsson, S. Stahl, and M. Uhlen. 1996. Integrated production of human insulin and its C-peptide. Journal of biotechnology. 48:241-250.
Thim, L., M.T. Hansen, K. Norris, I. Hoegh, E. Boel, J. Forstrom, G. Ammerer, and N.P. Fiil. 1986. Secretion and processing of insulin precursors in yeast. Proceedings of the National Academy of Sciences of the United States of America. 83:6766-6770.
Thompson, G.A., W.R. Hiatt, D. Facciotti, D.M. Stalker, and L. Comai. 1987. Expression in plants of a bacterial gene coding for glyphosate resistance. Weed Sci. 35:19-23.
Walmsley, A.M., and C.J. Arntzen. 2000. Plants for delivery of edible vaccines. Current opinion in biotechnology. 11:126-129.
Continue Reading

Please join StudyMode to read the full document

You May Also Find These Documents Helpful

  • Estimating Population Size Using Mark and Recapture Method Essay
  • Mark Essay
  • Essay on Tadpole Population – Mark and Recapture
  • Essay on Capture Recapture Method
  • mark Essay
  • Mark Essay
  • Marks Essay
  • The Mark Essay

Become a StudyMode Member

Sign Up - It's Free