Preview

Light Reaction Lab

Good Essays
Open Document
Open Document
477 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Light Reaction Lab
Light Reactions Lab
This lab explores the effect of light intensity and light wavelength on photosynthesis. The quantity of energy [ATP] produced will change depending upon on these parameters.
Use "Johnson Explorations: Photosynthesis" located at http://www.mhhe.com/biosci/genbio/biolink/j_explorations/ch09expl.htm Examine the diagram before clicking "skip intro" so you have an idea what is going on. The animation show the movement of photons, electrons, water, protons, ATP etc. relating to the ETC of a thylakoid.
Procedure: Use the simulation to determine how wavelength and light intensity affect the rate of photosynthesis [measured by ATP production]. You are dealing with two variables you must keep one variable constant while manipulating
…show more content…
Introduction: Describe briefly the light dependent reactions and propose a hypothesis to answer the experimental question.
2. Procedure: Briefly describe the simulation, and the method for obtaining your data.
3. Data: Include data tables for the simulation for both changes of intensity and changes of wavelength. More than one table is probably required.
4. Graphs showing how the percentage of ATP changed as a result of changes in wavelength and intensity . Use a spreadsheet or graphing program of your choice.
3. Conclusion: Use your data to answer the experimental question. Answer clearly how light wavelength affects the reaction, and how light intensity affects the reactions. Offer an explanation of the results, taking into account the principles of photosynthesis and the light reaction.
Grading Rubric Poor (1 pts) Satisfactory (2 pts) Good ( 3 pts)
Introduction Explanation of light dependent reaction lacking and/or hypothesis missing. Offers explanation of the reaction, some parts vague or incorrect. Hypothesis unclear or does not answer experimental question fully. Offers good explanation of the the light dependent reaction, includes how light is used to produce ATP and NADPH. Proposes hypothesis to answer experimental

You May Also Find These Documents Helpful

  • Good Essays

    Photosynthesis Lab

    • 1012 Words
    • 5 Pages

    AIM: to investigate the effect of light intensity (lux) on the amount of oxygen produced (number of bubbles)…

    • 1012 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    The following report is about the factors which can affect the pathways and the the rate of photosynthesis in plants. This report will cover what photosynthesis is , the factors which may affect photosynthesis in working poorly, light independant and dependant reaction. An investigation will be carried out which will include the factors that affect photosynthesis.…

    • 1319 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    Chloroplast Lab

    • 503 Words
    • 3 Pages

    DPIP will be used to determine the rate at which the cholorplasts are being reduced. The spectrophotometer will establish the wavelength of light that penitrats the chloroplast solution in turn determining the amount of electrons reduced. In the dark reactions, the spectrophotometer will measure the amount of light passing through a darker solution of DPIP and chloroplasts. In the light reactions, the lighter solution, caused by reduction of the chloroplasts, will allow a larger amount of light to pass through to the photocell of the spectrophotometer. Thus, the spectrophotometer will prove wheter the light or dark reactions affect the rate of photosynthesis in chloroplasts. We will also be using a reference solution made of water, phosphate buffer, and active chloroplasts. The purpose of this solution will be used to set the transmittance level for the experiment. The control solution, which is different than the reference solution, is comprised of water, phosphate buffer, and DPIP. It will be…

    • 503 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Photosynthesis Lab Report

    • 874 Words
    • 4 Pages

    Photosynthesis and cellular respiration are often mistaken as the same thing. Although they are similar in many ways, photosynthesis and cellular respiration are the exact opposite of each other. Not figuratively, but literally the reverse (Photosynthesis). They incorporate the others products while adding some outside energy to create a never ending cycle. This brings us to the photochemical and biochemical reactions of photosynthesis and cellular respiration. In an ordinary photochemical reaction, carbon dioxide, water and light energy produce glucose and oxygen. These products transfer to the biochemical reaction, where light…

    • 874 Words
    • 4 Pages
    Better Essays
  • Good Essays

    Light Dependent Reactions

    • 660 Words
    • 3 Pages

    During the Light Dependent Reactions, activity occurs within the thylakoids of the chloroplast. NADP+ accepts two high energy electrons and an H+ ion and then converts into NADPH. This process ultimately traps some of the sunlight in chemical form. Then, NADPH is able to carry the energy it absorbs to the rest of the cell. The reaction produces O2 gas and converts ADP to ATP and NADP+ to NADPH. First, pigments in Photosystem II absorb light, which is then absorbed by electrons, which are then passed along the Electron Transport Chain. Chlorophyll loses an electron, but interestingly, those missing electrons are replaced through enzymes in the thylakoid membrane, which divide H20. Secondly, electrons move through the Electron…

    • 660 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    the simulator to determine how each of the factors affects the rate of photosynthesis. …

    • 553 Words
    • 3 Pages
    Satisfactory Essays
  • Better Essays

    The purpose of this lab is to observe how influential light is to the construction and/or use of CO2 and O2 amongst plants. This can be examined by experimenting the effects of plants under both light and no light.…

    • 1015 Words
    • 5 Pages
    Better Essays
  • Good Essays

    Introduction/Background: Plants use light to turn carbon dioxide into sugars necessary for growth, a process called photosynthesis. Sunlight is a full spectrum of different wave lengths of light. Seen through a prism, this spectrum turns into bands of color: red, orange, yellow, green, blue, indigo and violet.1 However, changing the wavelength of light may shorten this process even more.2 Many of the results from other experiments have shown that this differs from plant to plant.3 In a previous experiment, done by Hamaker K., results have shown that vines under green light have shown more growth than vines under “white light”.4 However, an experiment done by Morton J. L., lima beans actually grow the best in “white light” and the poorest in green light.5 The conclusion is that different wavelengths of light in the visible spectrum have varying effects on the growth of photosynthetic plants.6 The purpose of this experiment is to see if different wavelengths of light affect the growth of raphanus sativus.…

    • 1029 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Red wavelengths will increase the rate of photosynthesis to a greater degree than blue or green wavelengths and thus will be the most optimal for plant growth. This hypothesis will be tested under laboratory conditions using silver beet extracts and DCPIP under different wavelengths and assessing the photosynthetic qualities using a spectrometer at 605nm. Photosynthesis is the process by which the suns visible light (electromagnetic radiation) is utilised by organisms with photosynthetic abilities (pigments called chloroplasts) as a chemical energy source. Living chloroplasts can be readily isolated from live plant tissue and used to investigate photosynthesis (Lilley et al. 1975; Halliwell 1984). Photosynthetic organelles are Eukaryotic and are fundamental for the survival of not only the organisms themselves, but the entire ecosystem around them as well. After the electromagnetic radiation is completely absorbed by the pigments, the electron transport chain (ETC) becomes a channel for excited electrons to pass through, capturing ATP and NADPH (Hoober, 1948) s and NADPH are then used to fix CO2 into carbohydrates (Halliwell 1984). The flow of electrons through the ETC can be monitored using the chemical DCPIP (Paterson and Arntzen 1982), which preferentially accepts electrons before the final stage of the ETC (Figure 6.1). When DCPIP is oxidised (before it accepts electrons) it is blue, but once it is reduced (has gained electrons) it turns colourless. Therefore when DCPIP is mixed with live chloroplast, the rate of change in DCPIP colour is proportional to the amount of electrons flowing through the ETC, which is in turn proportional to photosynthetic activity. The colour (and hence absorbance) of DCPIP can be measured using a spectrophotometer.…

    • 621 Words
    • 2 Pages
    Good Essays
  • Satisfactory Essays

    Rate of photosynthesis

    • 385 Words
    • 2 Pages

    Factors that could affect the rate of photosynthesis could be the duration of wavelength, high light intensity, or even air pollutants. The topic we researched was ‘High Light Intensity”. High light intensity happens beyond saturation point. Light intensity reduces the rate of photosynthesis. This affect is called solarization. It can…

    • 385 Words
    • 2 Pages
    Satisfactory Essays
  • Better Essays

    It is predicted that as the intensity of light increases, so will the rate of photosynthesis and the amount of oxygen produced.…

    • 3533 Words
    • 15 Pages
    Better Essays
  • Good Essays

    The goal of this study is to learn how the colour of light affects photosynthesis, to identify the effects of different coloured lights on plant growth, and to identify the potential use of different light colours as a growth regulator.…

    • 693 Words
    • 3 Pages
    Good Essays
  • Good Essays

    In 1905, Frederick Blackman, a British physiologist, concluded that photosynthesis, the capture of light and conversion of it into chemical energy is a two-way process, of which only one uses direct light to trap radiant energy. In the Hill Reaction activity, our group will be focusing directly on the light dependent reaction. The overall purpose of this experiment is to try to prove the hypothesis that this reaction does produce compounds that have the energy of light. The first of the two major reactions is referred to as the light reactions because they require light and are light dependent. These reactions are responsible for trapping radiant energy. The second reaction is concerned with using energy (light not required) captured by light…

    • 956 Words
    • 4 Pages
    Good Essays
  • Good Essays

    A previous lab in which we conducted, tested whether or not the light intensity had an effect on the rate of photosynthesis. We concluded that light intensity did possess an effect on the rate of photosynthesis – the closer the experimental plant units were to the light source, the more experimental plant units exhibited the effect of gas exchange in photosynthesis.…

    • 1917 Words
    • 8 Pages
    Good Essays
  • Good Essays

    Photosynthesis Lab

    • 1665 Words
    • 7 Pages

    An Experiment to Test the Effect of Temperature on the Rate of Photosynthesis in Green Algae…

    • 1665 Words
    • 7 Pages
    Good Essays