Light Blue Lab Report
Draw lines to represent where the bands will be.
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*light blue is 1 band
Experiment The group first designed the materials to create and hold the .75 agarose gel and experiment. Everything except the comb was made of ¼ inch Acrylic Plexiglass. The glass helped the group see what was going on in the experiment. The comb was 3D printed to be more accurate. One material was a casting tray which held the Gel in future steps. The casting tray had to hold at least 50 mL of liquid. They didn’t want the casting tray to hold much more than 50mL or else they’d be wasting materials. The group sealed two sides of the casting tray to the base with Bostik All Weather Sealant. The …show more content…
The .75 agarose gel is used so scientists can see when DNA runs through it. They mixed 45 mL of the TAE buffer with .75 grams of agarose powder. The group used TAE buffer rather than water because the TAE buffer is a salt based liquid. It contains a mixture of Tris base, acetic acid and EDTA. This means it is positively charged, and when electricity is run, the TAE buffer guides the electricity from negative to positive, which will be crucial for a future step. This happens because two valence electrons release, and move towards the positive charge. The group then boiled the mixture and then let it cool to 80 degrees Fahrenheit. The group then poured the mixture into the casting tray making sure the teeth of the comb reached the .75 agarose gel. Once the .75 agarose gel hardened, the group put it in the fridge overnight. Then, they took the taped sides off the .75 agarose gel. This is so electric current could run through the .75 agarose gel without being stopped by the wall. The group also took the comb out, that way there were wells to insert DNA …show more content…
Each DNA fingerprint should have several bands. The ladder would have been used as known DNA lengths to compare the DNA of the crime scene and suspects. The groups experiment did not go completely smoothly for a few major reasons. To begin with, each DNA fingerprint had no more than one band. This made it impossible to figure out who committed the crime because there was no available comparison to the crime scene DNA. Next, suspect two had no visible DNA. This major error made it impossible to observe the DNA fingerprint of that suspect. This caused the group to have a missing sample, and crucial information.
6,400
5140
4600
4310
4240
4160
3820
2880
2520
2500
2000
*light blue is 1 band
Experiment The group first designed the materials to create and hold the .75 agarose gel and experiment. Everything except the comb was made of ¼ inch Acrylic Plexiglass. The glass helped the group see what was going on in the experiment. The comb was 3D printed to be more accurate. One material was a casting tray which held the Gel in future steps. The casting tray had to hold at least 50 mL of liquid. They didn’t want the casting tray to hold much more than 50mL or else they’d be wasting materials. The group sealed two sides of the casting tray to the base with Bostik All Weather Sealant. The …show more content…
The .75 agarose gel is used so scientists can see when DNA runs through it. They mixed 45 mL of the TAE buffer with .75 grams of agarose powder. The group used TAE buffer rather than water because the TAE buffer is a salt based liquid. It contains a mixture of Tris base, acetic acid and EDTA. This means it is positively charged, and when electricity is run, the TAE buffer guides the electricity from negative to positive, which will be crucial for a future step. This happens because two valence electrons release, and move towards the positive charge. The group then boiled the mixture and then let it cool to 80 degrees Fahrenheit. The group then poured the mixture into the casting tray making sure the teeth of the comb reached the .75 agarose gel. Once the .75 agarose gel hardened, the group put it in the fridge overnight. Then, they took the taped sides off the .75 agarose gel. This is so electric current could run through the .75 agarose gel without being stopped by the wall. The group also took the comb out, that way there were wells to insert DNA …show more content…
Each DNA fingerprint should have several bands. The ladder would have been used as known DNA lengths to compare the DNA of the crime scene and suspects. The groups experiment did not go completely smoothly for a few major reasons. To begin with, each DNA fingerprint had no more than one band. This made it impossible to figure out who committed the crime because there was no available comparison to the crime scene DNA. Next, suspect two had no visible DNA. This major error made it impossible to observe the DNA fingerprint of that suspect. This caused the group to have a missing sample, and crucial information.