Larvicidal Effect Of Sampaguita Jasminum Sambac

Topics: Mosquito, Dengue fever, Aedes Pages: 18 (525 words) Published: April 5, 2015
Larvicidal effect of Sampaguita
(Jasminum sambac) leaf extract
on Mosquito (Aedes aegypti) larvae
The Researchers:
Clifford Cruz
Joseph Guillermo
Raymart Leyson
Daniel Manzanilla
Samantha Bismonte
Glory Tolentino

INTRODUCTION
Background of the Study
Endemic in more than 100 countries

cases of dengue fever
doubled in Metro Manila

INTRODUCTION
Statement of the Problem
• Is there a significant difference in the population of
the larvae of mosquitoes between the different
treatments?
• Is there a significant difference in the effectiveness
of different concentrations of Sampaguita Leaf
Crude Extract against the positive and negative
control?

INTRODUCTION
Significance of the Study
285 DEATHS of children between 1 and 9 years of age

Dengue cases still continue to be prevalent
search for more efficient and easier ways of eliminating mosquito larvae

eliminating the mosquito larvae before they could grow and
become infectious

INTRODUCTION
Significance of the Study

Table 1.1 Age distribution of Dengue cases in the Philippines (to September 24 2011) CFR: Case Fatality Rate

INTRODUCTION
Scope and Limitations of the Study

REVIEW OF RELATED LITERATURE
Alkaloids in making a larvicide
alkaloids

By­product of plant­
metabolism

larvicide

Chemical used for killing 
larvae

methanol extract of Piper longum fruit was active
against mosquito larvae Culex pipiens pallens
Pipernonaline: 
piperidine alkaloid

REVIEW OF RELATED LITERATURE
Dengue as an epidemic disease
Symptoms starts 3 to 4 days after
infection

Warmer  Dengue hemorrhagic fever
climates (severe form of dengue)
• PLASMA LEAKAGE

Philippines: largest number of
deaths in 2013 at 306 halfway
through the year

REVIEW OF RELATED LITERATURE
Aedes aegypti as a Dengue vector
Water
• Fresh
• Brackish
• Clear, turbid, or
polluted
 Except with high
salt concentration

female mosquitoes 
blood feed primarily on 
humans
 Incubation of 7­14 
days
 Salivary glands
 Larval stage: highly 
susceptible

REVIEW OF RELATED LITERATURE
Government Proposed Solutions to Dengue

REVIEW OF RELATED LITERATURE
Larvicide as one of the solutions to eradicate
Dengue
o Biological insecticides
o controlling the mosquito larvae before they leave
the water

Most 
effective

Most 
economica
l

Safest 
method

REVIEW OF RELATED LITERATURE
Sampaguita as larvicide source
Phytoconstituents
    Alkaloids
    Tannin
    Flavanoid
    Steroid
   Glycoside
   Terpenes
   Resin
   Salicylic acid

 Jasminum sambac
        +
        +
        +
        +
        +
        +
        +
        +

MATERIALS AND METHODS
Preparation of Sampaguita Leaves
• Random picking
• leaves were washed and dried for 1 week
• identified at the National Museum

MATERIALS AND METHODS
Preparation of Extracts






340 grams
Wiley Mill: pulverized
Soaked for 48 hours
Rotary Evaporator: concentrated
Water bath: to obtain semi-solid extract

MATERIALS AND METHODS
Preparation of Test Organisms
• Twenty larvae of the third instar level
were placed in cups for one set up.
• six replicates
• Total amount of larvae used: 1200

MATERIALS AND METHODS
Preparation of Larvicidal Properties
• Complete Randomized Design
• 20 larvae each cup
• Two control groups shall be randomized
and replicated six times
• In total, there are two control groups and
five experimental groups in the study

DISCUSSION

• Analysis of Variance (ANOVA)
• F-Value = 10.1837

• 8000 ppm of sampaguita leaf
crude extract is most effective

CONCLUSIONS AND RECOMMENDATIONS
Summary of Results
• larvicidal activity as concentration: time of
exposure
Conclusions
• there is a significant difference in the
effectiveness of different levels of apple seeds
between the five experimental set-ups in
which different amounts of the Sampaguita
(Jasminum sambac) crude leaf extract...
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