Lab Write Up 1
Whitney Shepard
BIOL9999
1/26/2015
Microbes in the Environment
Abstract
Microorganisms are plentiful and widespread in the environment. In this lab, we undertook to determine the differences in the agars being used and the different colony count observed. After taking four different samples of microbes from the environment and swabbed them in two different plates one with nutrient agar and the other with sabouaud dextrose agar. After the microbes had incubated for 48 hours no results were discovered from the swabs we had taken from the environment. This lab further investigates the different swabs from the environment other group got and the different results discovered from the agars being used and the colony count observed.
Introduction
The natural environment is populated with many microorganisms suspended in the air, in water, and associated with other organisms. All surfaces in the lab and in the environment are potential source of contamination. In order to examine microorganisms without the use of a microscope, they must be given the proper condition and nutrients to flourish into colonies. In this lab, microorganisms were taken and cultivated from four different sources. For each of these, there were two different medias used – nutrient agar and sabouraud dextrose agar. The intent is to determine the differences in the agars being used and the different colony count observed.
Materials and Methods
Initially, two plates were gathered one was nutrient agar (NA) and the other was sabouraud dextrose agar (SDA). Then gathered two packs of cotton tipped applicators. Then a magic marker was used to label each plate making quadrants numbering each one. Also the name and date was written on the edge of the plate. In the NA plate Whitney swabbed her left index finger in the first quadrant. Then washed her hands. In the second quadrant Whitney took her clean left index finger and swabbed it in there. In the SDA plate Adrianna swabbed her right index finger in
BIOL9999
1/26/2015
Microbes in the Environment
Abstract
Microorganisms are plentiful and widespread in the environment. In this lab, we undertook to determine the differences in the agars being used and the different colony count observed. After taking four different samples of microbes from the environment and swabbed them in two different plates one with nutrient agar and the other with sabouaud dextrose agar. After the microbes had incubated for 48 hours no results were discovered from the swabs we had taken from the environment. This lab further investigates the different swabs from the environment other group got and the different results discovered from the agars being used and the colony count observed.
Introduction
The natural environment is populated with many microorganisms suspended in the air, in water, and associated with other organisms. All surfaces in the lab and in the environment are potential source of contamination. In order to examine microorganisms without the use of a microscope, they must be given the proper condition and nutrients to flourish into colonies. In this lab, microorganisms were taken and cultivated from four different sources. For each of these, there were two different medias used – nutrient agar and sabouraud dextrose agar. The intent is to determine the differences in the agars being used and the different colony count observed.
Materials and Methods
Initially, two plates were gathered one was nutrient agar (NA) and the other was sabouraud dextrose agar (SDA). Then gathered two packs of cotton tipped applicators. Then a magic marker was used to label each plate making quadrants numbering each one. Also the name and date was written on the edge of the plate. In the NA plate Whitney swabbed her left index finger in the first quadrant. Then washed her hands. In the second quadrant Whitney took her clean left index finger and swabbed it in there. In the SDA plate Adrianna swabbed her right index finger in