Lab: The Effect Of Temperature On Enzyme Activity

Topics: Catalysis, Chemical reaction, Enzyme, Oxygen, Measurement, Catalase / Pages: 8 (1769 words) / Published: Mar 29th, 2016
Part B: Practical Report

The Effect of Temperature on Enzyme Activity

Aim: To investigate how temperature effects the enzyme catalase.

Hypothesis: If the temperature of water is increased then the enzyme will react quicker to form oxygen and water, when compared to cold water.

Purpose: To design and conduct a plan of a practical about the effects of temperature on enzymatic activity with a partner.

Introduction: An enzyme is a protein, which speeds up a specific chemical reaction without altering the products of the reaction and without being altered itself (LeCornu and Diercks, 2011). Enzymes catalyse a reaction by lowering the activation energy (LeCornu and Diercks, 2011). A number of factors may change an enzyme’s environment such
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Gather materials and rinse the glassware with distilled water and dry with a paper towel.

3. Pour up to 250ml of cold water at 27 °C in each of the three beakers. If the water is too cold, set it aside for a short time so the temperature will drop to 27 °C, repeatedly check the thermometer in the water (it must be left in there for at least 10 sec) to make sure it is the right temperature. If the water is too warm add more cold water. There should only be 250ml water in each beaker so pour out excess water if any.

4. Label measuring cylinders as 1, 2 and 3. Set out beakers next to each other in a straight row and record the temperature of the water in the results table. Check temperatures to ensure they are all the same using the thermometer. Place each measuring cylinder in each water-filled beaker.

5. Pour 81 ml of hydrogen peroxide into an empty beaker and label it ‘Hydrogen Peroxide’ to clarify its contents. Get a dripper bottle of detergent and transfer 9 ml of hydrogen peroxide from the beaker using a dropper and place the amount in a small measuring tube. Put two equal drops (as close to equal as possible) of detergent along with the 9ml of hydrogen peroxide in the small measuring tube in each measuring
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This may have contaminated the solution, which would result an increase or decrease of catalase activity. A parallax error may have occurred when measuring the amount of foam produced from each reaction, which decreased the precision of the results. Due to reaction time when using a timer the exact rate of reaction of the catalase activity cannot be determined. The time the liver reacts with the solution varied with the sample of 3 for each temperature. Because the reaction initiates as soon as the reactants make contact catalase activity would be higher than the true value in the results. Another possible error is that the room temperature changes therefore the temperature of the water cannot be kept constant with each trail and produces unreliable

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