Lab Report
Introduction
In unit 7.3 the experiment tested the ability of lactase to specifically bind and interact with lactose compared to maltose. In unit 7.4 the experiment tested the role, if any, that metal ions have on the activity of lactase. My hypothesis for unit 7.3 was knowing that lactase is specific for lactose, lactose will separate into galactose and glucose, as maltose will not change (153-155). Lactase should like lactose. For unit 7.4 my hypothesis was that EDTA will remove the ions, and will not work.
Materials/Methods
For unit 7.3 the experiment needed 2 microfuge tubes one labeled "L" for lactose and the other labeled "M" for maltose. The experiment also called for a total of 3 clean plastic pipettes. With one pipette add milk up to the 0.5 line of the lactose tube. With the second clean pipette add maltose solution up to the 0.5 line of the maltose tube. With the third clean pipette, add lactase solution to each tube, until the level of mixture in each tube comes up to the 1.0 line of the tube. Place both tubes in the 40 degrees celsius water bath and incubate them for 10 minutes. The other thing needed is glucose strips. After the tubes have been incubated for 10 minutes, place a glucose strip in each tube for one second. Let it sit for thirty seconds, then compare to the chart provided. For unit 7.4 the experiment called for two microfuge tubes. One labeled "control" and label another one "EDTA". Add 1.25 mL of EDTA to the EDTA tube, add 1.25mL of distilled water to the control tube. Add 3 drops of milk to each tube, invert the tube and let sit for 1 minute. Then add 3 drops of lactase solution to each tube. Then place both tubes in the 40 degrees celsius water bath and leave them for 10 minutes. After the 10 minutes are up, place the glucose strip in each tube and let the strips sit for 30 seconds. Then compare to the charts provided.
Results
In 7.3 after 10 minutes, the results came to be 500 mg/dL of glucose in the lactose tube and 1000
In unit 7.3 the experiment tested the ability of lactase to specifically bind and interact with lactose compared to maltose. In unit 7.4 the experiment tested the role, if any, that metal ions have on the activity of lactase. My hypothesis for unit 7.3 was knowing that lactase is specific for lactose, lactose will separate into galactose and glucose, as maltose will not change (153-155). Lactase should like lactose. For unit 7.4 my hypothesis was that EDTA will remove the ions, and will not work.
Materials/Methods
For unit 7.3 the experiment needed 2 microfuge tubes one labeled "L" for lactose and the other labeled "M" for maltose. The experiment also called for a total of 3 clean plastic pipettes. With one pipette add milk up to the 0.5 line of the lactose tube. With the second clean pipette add maltose solution up to the 0.5 line of the maltose tube. With the third clean pipette, add lactase solution to each tube, until the level of mixture in each tube comes up to the 1.0 line of the tube. Place both tubes in the 40 degrees celsius water bath and incubate them for 10 minutes. The other thing needed is glucose strips. After the tubes have been incubated for 10 minutes, place a glucose strip in each tube for one second. Let it sit for thirty seconds, then compare to the chart provided. For unit 7.4 the experiment called for two microfuge tubes. One labeled "control" and label another one "EDTA". Add 1.25 mL of EDTA to the EDTA tube, add 1.25mL of distilled water to the control tube. Add 3 drops of milk to each tube, invert the tube and let sit for 1 minute. Then add 3 drops of lactase solution to each tube. Then place both tubes in the 40 degrees celsius water bath and leave them for 10 minutes. After the 10 minutes are up, place the glucose strip in each tube and let the strips sit for 30 seconds. Then compare to the charts provided.
Results
In 7.3 after 10 minutes, the results came to be 500 mg/dL of glucose in the lactose tube and 1000
Cited: Reece, J. (2005). Biology. (9ed., Vol. 1, pp. 153-155). San Francisco, California: Pearson education.