Effect of enzyme concentration on rate of reaction
Research question: is there a relationship between catalase concentration and the rate of reaction?
Hypotheses: if the enzyme concentration is increased then the rate of reaction will increase. If the enzyme concentration is decreased then the rate of reaction will decrease.
Independent: % catalyze concentration (
Dependent: rate of reaction (mm/s)
Mortar and pestle
Chicken liver (or any other liver)
Sterile transfer pipet (Dropper)
Sterile weigh boat
Calculate mass of chicken liver needed for 1ml, 5ml, 10ml, 15ml, and 20ml catalyzes concentration mass to volume concentrations. In other words: 1% = .
15ml and H2O x = 0.15 g liver.
This means 0.15g of liver for each percent of catalyze concentration. Overall I need 2.25g of chicken liver because I will conduct each percent with 3 trials.
Measure 0.15g of chicken liver for 1% catalyzes concentration using electronic balance and a sterile weigh boat Grind the chicken liver until little to no clumps are visible Measure 15 ml of distilled water using graduated cylinder and transfer to mortar and pestle containing the chicken liver Using the sterile transfer pipet, transfer 1ml of hydrogen peroxide to each test tube Transfer 5ml of catalase concentration mixture to a different test tube Using a ruler, align the test-tube parallel to it
Start timer as enzyme concentration is being poured into the 1ml hydrogen peroxide Record each height of bubble foam (oxygen) formation using a ruler. Repeat steps 3-10 for 5%, 10%, 15%, and 20%
(make sure to you have 3 trials for each %)
To Control: add distilled water to 1 ml Hydrogen peroxide
When I need to measure:
Concentration of liver
Temperature of peroxide and water...
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