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INTRODUCTION There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose, Indole Production, and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous in his stool. The patient had no pain but mild discomfort during bowel movements. The doctor noted inflammation of the lining of the rectum. The doctor removed a tissue sample for examination and referred the patient to a gastroenterologist for a barium xray and colonoscopy. While utilizing the methods stated above, the bacterium found was a facultative anaerobe due to the Gram Negative Rods, fermentation, and the production of indole. The specific Gram Negative Enteric Bacilli was Shigella dysenteriae. There are many reasons for knowing how to control bacterial growth as well as such as antimicrobial drugs, which assist the body’s defenses against pathogens. Microorganisms are capable of causing infection or spoilage. Controlling bacterial growth is important in poultry farm businesses that use equipment to decontaminate processing plant fluids before disposal. During quality control and safety evaluations, bacteria growth that needs to be controlled can be found. While controlling bacterial growth, clusters of Gram Positive Cocci were found. It was found some ways to control bacterial growth were disinfection, sterilization, antisepsis, sanitization, moist and dry heat, filtration, and radiation; just to name a few. The specific method used for control depends on various circumstances.

PROCEDURE First, you want to place your test tubes on the bench; one being an unknown labeled as GrowthLab1 and the other is the medium. Specific biochemical tests will be performed



References: 1. Bauman, Robert W. Microbiology with Diseases by Taxonomy. Third Edition. San Francisco: Benjamin Cummings publishing, 2011 2. Intuitive Systems, Inc. Virtual Unknown Microbiology Internet Edition. Version 4.0.8.2. Intuitive Systems Inc., 1999

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