Lab Report

Topics: Escherichia coli, Bacteria, Urinary tract infection Pages: 4 (1053 words) Published: April 18, 2008
March 31,2008

Lab Report-Escherichia coli

The purpose for this lab report was to identify and inform of an unknown bacteria that has been causing a patient to have lower abdominal and pelvic pain. To obtain the identification of this unknown bacterium, several biochemical tests needed to be performed in order to prescribe the correct medication to treat and cure the symptoms. Introduction

In a lab today, I am to identify an unknown bacterium that is causing a patient lower abdominal pain. There are six different organisms that the unknown bacteria could be. They are Eschericha coli, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aerogenosa, and Salmonella typhimurium. I will be using several different types of biochemical testing. I am using this type of testing because it differentiate and sometimes identify microorganisms based on specific biochemical characteristics. A gram stain preparation was done first to confirm that the unknown bacterium was gram negative. From here a TSA slant and a TSA plate (for backup) was inoculated with the bacteria and then incubated at 37ºC between 24-48 hrs. Afterwards, they were observed for growth and used to perform a series of eight different tests to identify the unknown.

Material and Methods
Eight different prepared types of media were provided to use to identify the unknown bacterium. They were a Gelatin Tube, MR Tube(Methyl Red), VP Tube(Voges-Proskauer) Urea Tube, SIM Tube(Sulfur Indole Motility), Citrate Slant, TSIA Slant (Triple Sugar Iron Agar) and Oxidase. Each one of the tubes and slants were inoculated using either the streaking or stabbing technique with one of the colonies from the growth of the unknown bacterium. The Gelatin tube was incubated for up to a 48 hrs at 37ºC. It was afterwards place in 27ºC for about 1 hr to see if it solidifies. The MR Tube was incubated for 48 hrs at 37ºC. Afterwards 3 drops of...

References: Stout, M.A., L. Van Waasbergen, M.L. Badon. UTA Fall 2007 Microbiology Lab Handouts
Thomas, Blaine. Instructor Microbiology Lab. UTA of Arlington
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