Lab report 1 Virtual Lab: Classifying Using Biotechnology

Topics: Bacteria, DNA, RNA Pages: 3 (720 words) Published: September 25, 2013

Virtual Lab: Classifying Using Biotechnology
Worksheet
NOTE: As you read the information in the Microbiology Handbook, there may be some terms you are not familiar with – such as 16s ribosomal RNA and Polymerase Chain Reaction. Refer to your text to read background material explaining any terms or processes with which you are not familiar.

Record the results of your investigations of each unknown in Table 1 by completing the following steps: 1) Apply the stain to your first unknown slide and examine it under the microscope. 2) Record the shape of the bacteria, the arrangement of the bacteria, and the gram staining characteristics. 3) Analyze and record the G+C content of the sample by dragging the DNA tube that corresponds to the bacterial sample to the GC Content Measuring Apparatus. (Note: the identification of the DNA tubes may be confusing; the red tube belongs to sample #1, the blue tube belongs to sample #2, and the green tube belongs to sample #3) 4) Compare the slide to the pictures in the Microbiology Handbook, and record the name of the species you believe it to be. 5) Repeat these steps for sample #2 and sample #3.

6) Click reset to obtain 3 new bacterial samples to test, and repeat steps 1-5. 7) Continue to reset the slides and continue testing until you have identified each bacterial species described in the Microbiology handbook, as well as one unknown species.

Table 1:
ShapeArrangementGram + or Gram -GC ContentSpecies
BacilliIrregular rodsNeg~51%E. coli
CocciIrregular clustersPos.~25%Staph Aureus
CocciChainsPos.~30%Strep mutans
No matchNo match~50%E. coli
CocciPairsNeg.~75%Veilonella sp.
CocciCubical packages of 4-8 cellsPos.~45%Sarcina sp.
BacilliCurved rods with flagellumNeg.~35%Vibrio Cholerae Oval Cocci/bacilli Oval irregularNeg~67%Bordetella pertussis

Answer the following questions:
1. Which of the species you...
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