Lab

Topics: Germination, Seed, Radicle Pages: 3 (1313 words) Published: February 23, 2015
Toxicology Lab 
 
 
1. In  this investigation, a wide range of concentrations of  Sodium Chloride (NaCl) solution  were   created   and  the  effects  that  they  had  on  radish   seeds  were  tested.  This  ultimately  created  a  dose­response  experiment  in  which  it  was  detectable whether  or  not  radish  seeds  were  a  reliable  bioassay  for  the   toxicity  of  NaCl.  The  goal  of  this  experiment  was  to  determine  a  correlation  between  toxicity  and  seed  germination/radicle  length.  Our  data  indicated  that  seed  germination  decreased  as  concentration  increased,  demonstrated  by  our   dramatic  difference  between  the  percentage  of  seeds  germinated  in  our  control  group  (0%)  and  the  percentage  of  seeds  germinated  in  our  100%  solution  (33%).  It  additionally  indicated  that  radical  length  decreased as concentration increased, demonstrated by the difference between  the  average  length  of  14.5  mm  in  our  control  group  and the average length of .47 mm  in  our  100%  solution. Ultimately, it  was  determined  through  careful  procedure  that  as  toxicity increased, germination rate and radical length decreased.   

2. This  experiment   was  performed  to  judge  how  the  radish  seeds  would  respond  to  different concentrations of Sodium chloride (NaCl). This is significant because it serves  as  data  to  prove  whether  radish  seeds serve as a good  bioassay for the toxicity of this  particular  chemical  compound.  This  experiment  was  specifically  useful  to  our  knowledge  because  it  demonstrated  a  realistic  and  applicable  version  of  a  dose­response  curve.  We used a dose­response experiment because it enveloped the  exact  information  needed  to conclude  the  response  of  the radish  seeds  to  increasing  levels  of  toxicity.  Our  hypothesis  was  that  as  toxicity  would  increase,  the  rate  of  germination  and  the  radical  length  would   decrease.  We ...
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