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Lab 6: Cellular Respiration

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Lab 6: Cellular Respiration
AP Biology I
Investigation 6
AP Bio: Lab 6 Cellular Respiration
Introduction
Some knowledge that is needed before performing this lab are as follows: First of all, cellular respiration is the metabolic processes whereby certain organisms obtain energy from organic molecules. This process includes glycolysis, the Krebs cycle, and the Electron Transport Chain. Glycolysis is a process that takes place in te cytosol and it oxidizes glucose into two pyruvate. Glycolysis also makes ATP and NADH. The Krebs Cycle occurs in the mitochondria and this process takes the pyruvate and breaks it down into carbon dioxide. But it also produces 3 CO2, 1 ATP, 1 FADH2, and 4 NADH. The electron transport chain takes place in the inner mitochondrial membrane nd creates H+ gradients and 36 ATP from glucose (Campbell, 2008).
In procedure one, the purpose was to determine the affect the temperature has on both sprouted and nonsprouted peas. My hypothesis is that if the peas are geminated then they will show a higher amount of cellular respiration in comparison to nongerminated peas. Also, if the different types of peas are placed in the colder water then they will show a slower rate of respiration than they will in room temperature water (Carolina Biological, 2006).
In procedure two, the purpose was to see if the darkness of a plant would affect the amount of cellular respiration that occurred. My hypothesis was that if the lettuce was darker then it would show more cellular respiration than that of a lighter shaded plant.
Materials and Procedures
In the first procedure, we used 25 germinating peas, 25 nongerminating peas, 25 glass beads, 6 vials, 6 rubber stoppers, 6 flat metal washers, parafilm,1 tub of room temperature water, 1 tub of ice water, 2 thermometers, 6 pieces of nonabsorbent cotton, 6 pieces of absorbent cotton, 3 graduated cylinders, a beaker of 15% solution of KOH, two pieces of paper, and food coloring. The glass balls served as the control. The KOH absorbed the

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