Lab 2 Autosaved 
NAME: Samantha Cruickshank ID# 620040626
Name of lab partners: Krystal Ramsaroop, Kristin Sigh, Debbion Sylvester, Vallene Bernard
Demonstrator: Adanna
DATE (of lab session): Week 7 (Tuesday 3rd, 2015)
Course Code BIOL 1362
Title Of Lab: Estimation of Ascorbic acid
Aims:
To determine the mean ± S.D. ascorbic acid equivalence per 1mL of dye.
To determine the ascorbic acid content in mg per mL of canned orange juice.
Introduction:
Vitamin C is highly water-soluble and has both acidic and strong reducing properties. It occurs naturally in many animals and plants such as the plants of the fruits used in this lab. Vitamin C exist naturally in the L-ascorbic acid form. It is a strong reducing agent and when oxidative stress is present, it easily converts to the oxidized form which is. Vitamin C is an essential nutrient in humans because it functions as a cofactor in several vital enzymatic reactions. Humans do not synthesis vitamin C so need to obtain it from other sources such as juices and fruits. A deficiency of vitamin C would lead to scurvy in human. It has many benefits which include prevention of the common cold/heart diseases and strengthening of the immune system. The recommendation for vitamin C intake for humans is approximately 60 – 95 mg per day and a maximum intake of 2000 mg per day. A long term overdose of vitamin C may cause diarrhea, iron overload disorders and kidney stones. (Food Science and Technology Strand 2015) L-ascorbic acid L-dehydroascorbic acid
(Sánchez 2013) In this experiment, we use titration method (volumetric analysis) to determine the centration of vitamin C in packaged fruit juice samples such as the mango, apple, passion fruit and orange juices in this experiment. Titration is a commonly used method of quantitative analysis which can determine the concentration of a known analyte (reactant). In this case the reactant is vitamin C. A titrant (reagent - Dichlorophenol-indophenol) of known concentration
Name of lab partners: Krystal Ramsaroop, Kristin Sigh, Debbion Sylvester, Vallene Bernard
Demonstrator: Adanna
DATE (of lab session): Week 7 (Tuesday 3rd, 2015)
Course Code BIOL 1362
Title Of Lab: Estimation of Ascorbic acid
Aims:
To determine the mean ± S.D. ascorbic acid equivalence per 1mL of dye.
To determine the ascorbic acid content in mg per mL of canned orange juice.
Introduction:
Vitamin C is highly water-soluble and has both acidic and strong reducing properties. It occurs naturally in many animals and plants such as the plants of the fruits used in this lab. Vitamin C exist naturally in the L-ascorbic acid form. It is a strong reducing agent and when oxidative stress is present, it easily converts to the oxidized form which is. Vitamin C is an essential nutrient in humans because it functions as a cofactor in several vital enzymatic reactions. Humans do not synthesis vitamin C so need to obtain it from other sources such as juices and fruits. A deficiency of vitamin C would lead to scurvy in human. It has many benefits which include prevention of the common cold/heart diseases and strengthening of the immune system. The recommendation for vitamin C intake for humans is approximately 60 – 95 mg per day and a maximum intake of 2000 mg per day. A long term overdose of vitamin C may cause diarrhea, iron overload disorders and kidney stones. (Food Science and Technology Strand 2015) L-ascorbic acid L-dehydroascorbic acid
(Sánchez 2013) In this experiment, we use titration method (volumetric analysis) to determine the centration of vitamin C in packaged fruit juice samples such as the mango, apple, passion fruit and orange juices in this experiment. Titration is a commonly used method of quantitative analysis which can determine the concentration of a known analyte (reactant). In this case the reactant is vitamin C. A titrant (reagent - Dichlorophenol-indophenol) of known concentration
References: Advanced Instructional Systems, Inc. and the University of California, Santa Cruz . 2011. webassign. Accessed March 8, 2015. http://www.webassign.net/labsgraceperiod/ucscgencheml1/lab_14/manual.html. Dorothy J. VanderJagt, Philip J. Garry, and William C. Hunt. 1986. "Ascorbate in plasma as measured by liquid chromatography and by dichlorophenolindophenol colorimetry." Clinical Chemistry. June. Accessed March 10, 2015. http://www.clinchem.org/content/32/6/1004.long. Food Science and Technology Strand. 2015. " Determination of Vitamin C (Ascorbic Acid) Concentration in Fruit Juice ." Food Studies. Food Science and Technology Strand, March 08. Sánchez, José Luis Silencio Barrita and María del Socorro Santiago. 2013. "Antioxidant Role of Ascorbic Acid and His Protective Effects on Chronic Diseases." Oxidative Stress and Chronic Degenerative Diseases - A Role for Antioxidants. May 22. Accessed 03 10, 2015. http://www.intechopen.com/books/oxidative-stress-and-chronic-degenerative-diseases-a-role-for-antioxidants/antioxidant-role-of-ascorbic-acid-and-his-protective-effects-on-chronic-diseases.