Kreb Biology Lab Report

14-01-05

Cellular Respira8on

Upcoming events:
L3.1 {cell resp} – Jan 16/17th
Quiz Jan 22/23st (in lieu of write-up)

Mitochondrial

Cytoplasm (Cytosol)

Mitochondria

Membrane
• Nobel Prize in 1953 along with Fritz Albert Lipmann for the discovery of Coenzyme-­‐A. • An 8-­‐step process to further break down the Glucose into CO2

• Each step catalyzed by a specific enzyme. • It is a cycle because the product of step 8 is the reactant in step 1 (oxaloacetate). Substrate Level
Phosphorylation

1

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Electron Carriers (coenzymes)
H

Krebs Cycle

H

• The overall chemical equa%on is:

2 oxaloacetate + 2acetyl-­‐coA + 2ADP + 2P + 6NAD+ + 2FAD à 2CoA + 2ATP + 6NADH + 6H+ + 2FADH2 + 4CO2 + 2
The six carbon atoms have le[ as carbon dioxide molecules.

What’s leH of Glucose? • All that is preserved are 4 ATP (two from glycolysis and two from the Krebs Cycle) and 12 reduced coenzymes/electron carriers:

– 2 NADH from glycolysis

PART 1 – 2 NADH from pyruvate oxida%on

PART 2 – 6 NADH from the Kreb’s Cycle and

PART 3 – 2 FADH2 from the Kreb’s Cycle

PART 3 • Most of the energy from glucose will be produced in the next stage