KaylaWilson SC121 Unit 3 Assignment Lab
LABORATORY REPORT
Activity:
Blood Glucose Regulation
Name:
Kayla Wilson
Instructor:
Donald Cragen
Date:
10.29.2014
Predictions
Plasma glucose levels will be highest immediately after the meal (0 hr)
Plasma ketone levels will be highest before the meal (fasting)
Plasma insulin levels will be highest
1-3 hours after the meal
Plasma glucagon levels will be highest
1-3 hours after the meal
Materials and Methods
Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon
Independent Variable food and beverage intake
Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI
4. Why were physical activity and caffeine and alcohol intake the controlled variables?
They were the controlled variables because they were told not to drink anything containing caffeine or alcohold
5. What type of assay was used to measure plasma glucose and ketone levels?
Enzymatic Analysis
6. What type of assay was used to measure plasma insulin and glucagon levels? radioimmunoassay Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal
2 hr post meal
3 hr post meal
Sub. 1
5.2
6.4
6.3
5.6
4.7
Glucose (mmol/l)
Sub. 2
Sub. 3
5.3
5.3
6.3
6.5
6.3
5.5
4.6
6.4
5.6
4.7
average
5.3
6.4
Sub. 1
96
97
6.3
5.6
4.7
58
58
72
Laboratory Report/ Kayla Wilson/ Blood Glucose Regulation/ Donald Cragen/ 10.29.2014/ Page [1] of [4]
Ketones (µmol/l)
Sub. 2
Sub. 3
99
98
94
101
54
55
70
66
59
66
average
98
97
59
57
69
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
0 hours post meal
2. When was plasma glucose concentration lowest? If values are similar for several time points, then give range of times when plasma glucose concentration was lowest.
3 hours post meal
3.
Activity:
Blood Glucose Regulation
Name:
Kayla Wilson
Instructor:
Donald Cragen
Date:
10.29.2014
Predictions
Plasma glucose levels will be highest immediately after the meal (0 hr)
Plasma ketone levels will be highest before the meal (fasting)
Plasma insulin levels will be highest
1-3 hours after the meal
Plasma glucagon levels will be highest
1-3 hours after the meal
Materials and Methods
Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon
Independent Variable food and beverage intake
Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI
4. Why were physical activity and caffeine and alcohol intake the controlled variables?
They were the controlled variables because they were told not to drink anything containing caffeine or alcohold
5. What type of assay was used to measure plasma glucose and ketone levels?
Enzymatic Analysis
6. What type of assay was used to measure plasma insulin and glucagon levels? radioimmunoassay Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal
2 hr post meal
3 hr post meal
Sub. 1
5.2
6.4
6.3
5.6
4.7
Glucose (mmol/l)
Sub. 2
Sub. 3
5.3
5.3
6.3
6.5
6.3
5.5
4.6
6.4
5.6
4.7
average
5.3
6.4
Sub. 1
96
97
6.3
5.6
4.7
58
58
72
Laboratory Report/ Kayla Wilson/ Blood Glucose Regulation/ Donald Cragen/ 10.29.2014/ Page [1] of [4]
Ketones (µmol/l)
Sub. 2
Sub. 3
99
98
94
101
54
55
70
66
59
66
average
98
97
59
57
69
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
0 hours post meal
2. When was plasma glucose concentration lowest? If values are similar for several time points, then give range of times when plasma glucose concentration was lowest.
3 hours post meal
3.