Preview

Isolation and Molecular Characterization of Phosphate Solubilizing Rhizoplane Bacteria from Wheat (Triticum Spp)

Better Essays
Open Document
Open Document
956 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Isolation and Molecular Characterization of Phosphate Solubilizing Rhizoplane Bacteria from Wheat (Triticum Spp)
Synopsis on
TITLE:
Isolation and Characterization of Potential Phosphate Solubilizing Bacteria from Wheat (Triticum spp) of Bangladesh Introduction Wheat (Triticum spp.) is a cereal grain, originally from the Levant region of the Near East and Ethiopian Highlands, but now cultivated worldwide. In 2010 world production of wheat was 651 million tons, making it the third most-produced cereal after maize (844 million tons) and rice (672 million tons)(FAO,2010). In Bangladesh, wheat cultivation area and production were 388 hectares and 844 M. tons, respectively in the year 2007-2008 (BBS, 2008). The recommended doses of fertilizer for wheat are 100-120 Kg N ha-1, 50-60 Kg P2O5 ha-1 and 50-60 Kg K2O ha-1.
Phosphorus is one of the essential mineral macronutrients, which is required for maximizing the yield of crops, a part of several key plant structural compounds and as a catalytic compound involved in the conversion of numerous key biochemical reactions in plants. P is a vital component of DNA, the genetic "memory unit" of all living organisms, also a component of RNA, the compound that reads the DNA genetic code to build proteins and other compounds essential for plant structure, seed yield, and genetic transfer, a vital component of ATP, the "energy unit" of plants, which forms during photosynthesis and processes from the beginning of seedling growth through the formation of grain and maturity (Griffith, 2009).

However, a large portion of soluble inorganic phosphate applied to soil as chemical fertilizer is rapidly immobilized soon after application and becomes unavailable to plants (Dey, 1988; Yadav and Dadarwal, 1997). Plant root-associated phosphate solubilizing bacteria (PSB) have been considered as one of the possible alternatives for inorganic phosphate fertilizers for promoting plant growth and yield (deFreitas et al., 1997; Rodrı´guez and Fraga, 1999; Richardson, 2001; Vessey, 2003; Thakuria et al., 2004). Seed or soil inoculation with PSB is



References: Abd-Alla M. H. (1994), Phosphatases and the utilization of organic phosphorus by Rhizobium leguminosarum biovar viceae. Lett. Appl. Microbiol. 18, 294D296. BBS (Bangladesh Bureau of Statistics). 2008. Statistical Year Book. Stat. Div. Ministry Planning, Gov.People’s Repub. Bangladesh. Dhaka. pp.126-131. Bashan Y. and Holguin G. (1998), Proposal for the division of plant growth-promoting rhizobacteria into two classifications: Biocontrol-PGPB (plant growth promoting bacteria) and PGPB. Soil Biol. Biochem. 30, 1225D1228. Dey K. B. (1988), Phosphate solubilizing organisms in improving fertility status. In: Biofertilizers: Potentialities and Problems (Sen S. P. and Palit P., eds.). Plant Physiology Forum, Naya Prokash, Calcutta, pp. 237D248. Griffith D. B. 2009. Efficient fertilizer use – Phosphorus. Scientia agric., 433: 23-67. Mehta S. and Nautiyal C. S. (2001), An efficient method for qualitative screening of phosphate-solubilizing bacteria. Curr. Microbiol. 43, 51D56. Ryder M. H., Stephens P. M., and Bowen G. D. (1994), Improving Plant Productivity with Rhizosphere Bacteria. Commonwealth Scientific and Industrial Research Organization, Adelaide, Australia. Rodrı´guez H. and Fraga R. (1999), Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Adv. 17, 319D339. Richardson A. E. (2001), Prospects for using soil microorganism to improve the acquisition of phosphorus by plants. Aust. J. Plant Physiol. 28, 897D906. Vessey J. K. (2003), Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255, 571D586. Yahya A. and Al-Azawi S. K. (1989), Occurrence of phosphate-solubilizing bacteria in some Iraqi soils. Plant Soil 117, 135D141.

You May Also Find These Documents Helpful

  • Better Essays

    The use of soluble nitrogenous fertilizers in conventional farming, impacts the nutritional value of the food grown (Bacchus 2012, p.25). It leads to an increase in plant growth and development however a decreased allocation of resources towards the production of secondary metabolites (Mitchell & Chassy 2011). These include DNA, RNA, chlorophyll, amino acids and starch and phytochemicals of caffeine, isoflavonoids and phenolic antioxidants (Mitchell & Chassy 2011).…

    • 1507 Words
    • 6 Pages
    Better Essays
  • Good Essays

    2. Madigan, M.T., and J.M. Martinko. 2006. Brock biology of microorganisms. p. 707 and 781 11th ed. Prentice Hall, Upper Saddle River, NJ.…

    • 784 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    American River Diversity

    • 1365 Words
    • 6 Pages

    Using the Powersoil DNA extraction kits, one gram of soil will be used with six individual reagents to purify the sample of any impurities that might interfere with our results. The six reagents used are labeled as C1, C2, C3, C4, C5, and C6, each with their individual properties to unpurified unwanted material in our soil sample. From here, perform a serial dilution from the soil and water sample. This will be used for plating bacteria on agar to see potential colonies that are present in the samples. Colonies that arises from this sample can help us estimate the culturable bacteria (CFU's) found in the American…

    • 1365 Words
    • 6 Pages
    Powerful Essays
  • Satisfactory Essays

    Phosphorus Cycle Project

    • 516 Words
    • 3 Pages

    It is also one of the slowest biogeochemical cycles. Over long periods of rocks being weathered they begin to let out phosphate ions, after the phosphate ions are released they are distributed into the soil and water. Plants begin to take up the phosphate ions that were released by the rocks are are eaten by the animals. After the animals eat the plants that have the phosphate ions in them they eventually die and when they are decomposed the ions are released back into the soil. Phosphorus is needed for every living organism to grow and function. It is found in DNA and RNA materials and in ATP which is the energy stored in cells. Humans return the phosphorus into the earth from our waste. When we return the phosphorus back to the earth it cycles back through again and again…

    • 516 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Phosphorus exists in many different kinds of soil minerals and rocks. Phosphorus takes place in a slow process. Phosphate and other ions are released from rock as they break down through time into the soil. The phosphate turns into…

    • 858 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Soil Microbe Lab

    • 1259 Words
    • 6 Pages

    The objective of this soil microbe experiment is to isolate potential antibiotic-producing microbes in the soil that was taken from a local sample. To do so, two different samples of bacteria have been taken and placed onto agar plates (labelled A and B). As we allow each sample to grow, our objective will be to see what, if any, antibiotics develop and inhibit the growth of the bacteria. We predict that antibiotics will form on the agar plates, thus inhibiting the growth…

    • 1259 Words
    • 6 Pages
    Better Essays
  • Good Essays

    A common and widespread symbiosis occurs between terrestrial plants and fungi that colonize their roots; this is called "mycorrhizae.” Unlike pathogenic fungi that cause disease, mycorrhizal fungi benefit the plant in several ways. These fungi germinate from spores in the soil to form thin threadlike structures called hyphae, which grow into the roots of plants. Once the roots are colonized, the fungal hyphae grow out from the root to explore the soil beyond the reach of the roots, gathering essential mineral nutrients and transporting them into the plant, increasing its growth. In return, the plant provides carbohydrates as a food source for the fungus. Mycorrhizal symbiosis occurs in about 80 percent of all plant species. It is essential to many plants in low-nutrient environments because their roots alone are incapable of absorbing enough of some essential minerals such as phosphorus. The symbiosis is essential to the fungus because,…

    • 1005 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Having checked two Nutrient plates, signs of visible growth of two different bacterium were present. Due to the fact, that one type of bacteria didn’t appear to be fully…

    • 1749 Words
    • 7 Pages
    Better Essays
  • Good Essays

    Phosphorus is an essential macro-nutrient for plant growth and sustainable soil management. Phosphorus nutrition can be challenging in acidic soils (e.g. Queensland) and alkaline soils (e.g. South Australia). To achieve sufficient nutrition, the soil needs to have a relatively high pH, high organic matter content, and moist conditions. Specific soil orders such as Vertosols, Kandosols, Sodosols, Rudosols, and Calcarosols, lack the requirements for phosphorus nutrition. The availability of phosphorus heavily relies on soil pH; the pH range for highest availability is between 5.5 and 7.0, whereas, Queensland and South Australia are both out of this range.…

    • 1034 Words
    • 5 Pages
    Good Essays
  • Good Essays

    The phosphorus cycle includes the cycle of all the biologically important nutrients found in the natural minerals. These elements include iron, calcium, potassium found in the rock and soil minerals in the lithosphere. Over time a rock breaks down and releases phosphate (PO43-) and other ions which replenish phosphorus that is lost due to runoffs and leaching. The phosphate is absorbed by plants and turned into compounds that are moved through the food chain. Humans impact this cycle because we are using the phosphorus to make fertilizers, animal feeds, detergents or other products…

    • 645 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    The Phosphorus Cycle

    • 186 Words
    • 1 Page

    Phosphorus is a nutrient needed for humans animals and plants. This cycle is the slowest cycle through all the biogeochemical cycles. This cycle is an element of life.…

    • 186 Words
    • 1 Page
    Satisfactory Essays
  • Good Essays

    Phosphorus is usually present in natural waters as phosphate(Mcwelsh and Raintree, 1998). Phosphates are present in fertilizers and laundry detergents and can enter the water from agricultural runoff, industrial waste, and sewage discharge (Outwater,1996) . Phosphates, like nitrates, are plant nutrients (Phosphates, 1997). When too much phosphate enters a water, plant growth flourishes (Phosphates). Phosphates also stimulate the growth of algae which can result in an algae bloom(World Book Encyclopedia,1999). . Algae blooms are easily recognized as layers of green slime, and can eventually cover the water's surface. As the plants and algae grow, they choke out other organisms. These large plant populations produce oxygen in the upper layers of the water but when the plants die and fall to the bottom, they are decomposed by bacteria which use a lot of the dissolved oxygen in the lower layers (Phosphates). Bodies of water with high levels of phosphates usually have high biological oxygen demand (BOD) levels due to the bacteria consuming the organic plant waste and subsequent low dissolved oxygen levels(Hooper,1998).…

    • 831 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    All about ATP

    • 292 Words
    • 2 Pages

    The fact that ATP is Nature's 'universal energy store' explains why phosphates are a vital ingredient in the diets of all living things. Modern fertilizers often contain phosphorus compounds that have been extracted from animal bones. These compounds are used by plants to make ATP. We then eat the plants, metabolise their phosphorus, and produce our own ATP. When we die, our phosphorus goes back into the ecosystem to begin the cycle again...…

    • 292 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    pH and Microbial Growth

    • 279 Words
    • 2 Pages

    Followed lab procedure 29, “pH and Microbial Growth” in Benson’s Microbiological Applications: Laboratory Manual in General Biology, 12th edition.…

    • 279 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    Address Applied Biotechnology Research Group, School of Life Sciences, University of Westminster, United Kingdom Corresponding author: Keshavarz, Tajalli (T.Keshavarz@westminster.ac.uk) and Current Opinion in Microbiology 2010, 13:321–326 This review comes from a themed issue on Ecology and Industrial Microbiology Edited by Erick Vandamme Available online 12th March 2010 1369-5274/$ – see front matter # 2010 Elsevier Ltd.…

    • 4426 Words
    • 22 Pages
    Powerful Essays

Related Topics